Cell Counting Kit-8 (CCK-8) هو محلول جاهز للاستخدام من زجاجة واحدة يوفر قياسًا بسيطًا وسريعًا وموثوقًا وحساسًا لفعالية الخلايا وسميتها السمية لمجموعة متنوعة من المواد الكيميائية.

مزيج الماستر الخاص بـ SYBR Green qPCR Super Hot-Start هو كوكتيل جاهز للاستخدام في تقنية PCR الكمية.

مجموعة كيميلومينسنتية مبنية على اللومينول لكشف الأجسام المضادة المعبرة بالبيركسيداز (HRP) والمستضافات المرتبطة بها.

مخلب مثبط الفوسفاتاز I يثبط بشكل فعال الفوسفاتازات القلوية والبروتين فوسفاتاز المرتبطة بالأحماض الأمينية سيرين / ثريونين مثل PP1 و PP2A.

يستخدم مزيج مثبطات البروتياز في المستخلصات الخلوية أو المستخلصات النسيجية لزيادة استقرار البروتين.

Annexin V-FITC/PI Apoptosis Detection Kit is an apoptosis detection kit that uses FITC-labeled Annexin V protein to detect cell apoptosis levels. Annexin V is a Ca2+-dependent phospholipid-binding protein with a molecular weight of 35-36kD. It is widely distributed in the cytoplasm of eukaryotic cells and participates in intracellular signal transduction. In normal cells, phosphatidylserine (PS) is only distributed on the inside of the cell membrane lipid bilayer; in the early stages of apoptosis, phosphatidylserine is flipped over, and FITC-labeled Annexin V can interact with the phosphatidylserine flipped to the outside of the membrane. Serine specifically binds with high affinity to mark apoptotic cells. Apoptosis can be detected through flow cytometry, fluorescence microscopy or other fluorescence detection equipment. This kit also provides Propidium Iodide (PI) staining solution. Propidium iodide is a phenanthridine fluorescent dye that can bind to DNA. It can stain necrotic cells or cells that have lost their cell membrane integrity in the late stage of apoptosis, showing red fluorescence, while living cells and early apoptotic cells have Propidium Iodide. The phenomenon of dye rejection. After combined staining with Annexin V-FITC and PI, normal cells had almost no fluorescence (Annexin V-/PI-), early apoptotic cells showed green fluorescence (Annexin V+/PI-), and late apoptotic cells and necrotic cells showed green and red fluorescence (Annexin V+/PI+).

Annexin V-AF647/PI Apoptosis Kit is an apoptosis detection kit that uses AF647-labeled Annexin V protein to detect cell apoptosis levels. Annexin V is a Ca 2+ -dependent phospholipid-binding protein with a molecular weight of 35-36kD. It is widely distributed in the cytoplasm of eukaryotic cells and participates in intracellular signal transduction. In normal cells, phosphatidylserine (PS) is only distributed on the inside of the cell membrane lipid bilayer; in the early stages of apoptosis, phosphatidylserine is flipped over, and AF647-labeled Annexin V (Ex/Em:650/670nm) can interact with the phosphatidylserine flipped to the outside of the membrane. Serine specifically binds with high affinity to mark apoptotic cells with red fluorescence. Apoptosis can be detected through flow cytometry, fluorescence microscopy or other fluorescence detection equipment. This kit also provides a PI staining solution (Ex/Em:535/617nm). PI is a high-affinity nucleic acid stain that can only penetrate cells with damaged plasma membranes, making the nuclei of necrotic cells or late-stage apoptotic cells show red fluorescence, while live cells and early-stage apoptotic cells are resistant to PI staining. After co staining with Annexin V-AF647 and PI, normal cells showed almost no fluorescence (Annexin V-/PI-). Early apoptotic cells were Annexin V-AF647 positive (Annexin V+/PI -), while late apoptotic and necrotic cells were Annexin V-AF647 and PI positive (Annexin V+/PI+).

One-step TUNEL Apoptosis Detection Kit (Green, FITC) provides a fast and highly efficient method for detecting the degree of nuclear DNA fragmentation in cells and tissues during apoptosis, Ex/Em = 492/517nm. During apoptosis, some specific DNA endonucleases are activated, which cleave genomic DNA into multimers of 180~200bp fragments between nucleosomes, exposing 3'-OH ends at each DNA fragments terminal. In proliferating or non-apoptotic cells, there is almost no DNA fragmentation, resulting in minimal formation of 3'-OH ends. Terminal deoxynucleotidyl transferase (TdT) catalyze green fluorescent-marked dUTP label to the 3'-OH ends of fragmented DNA, which can be detected with fluorescence microscope. This kit can be used to detect cell apoptosis in frozen or paraffin sections, and can also in cultured adherent cells. This kit offers multiple components to accommodate various experimental needs: 1. Proteinase K (2mg/mL) is used for permeabilizing samples; 2. DNase I (2U/μL) and 10×DNase I Buffer are used to prepar e positive control samples; 3. 5×Equilibration Buffer, FITC-12-dUTP Mix, and TdT Enzyme are used to prepare the labeling solution.

One-step TUNEL Apoptosis Detection Kit (Orange, CY3) provides a fast and highly efficient method for detecting the degree of nuclear DNA fragmentation in cells and tissues during apoptosis, Ex/Em = 550/570nm. During apoptosis, some specific DNA endonucleases are activated, which cleave genomic DNA into multimers of 180~200bp fragments between nucleosomes, exposing 3'-OH ends at each DNA fragments terminal. In proliferating or non-apoptotic cells, there is almost no DNA fragmentation, resulting in minimal formation of 3'-OH ends. Terminal deoxynucleotidyl transferase (TdT) catalyze orange fluorescent-marked dUTP label to the 3'-OH ends of fragmented DNA, which can be detected with fluorescence microscope. This kit can be used to detect cell apoptosis in frozen or paraffin sections, and can also in cultured adherent cells. This kit offers multiple components to accommodate various experimental needs: 1. Proteinase K (2mg/mL) is used for permeabilizing samples; 2. DNase I (2U/μL) and 10×DNase I Buffer are used to prepar e positive control samples; 3. 5×Equilibration Buffer, CY3-dUTP Mix, and TdT Enzyme are used to prepare the labeling solution.

يعد Gsafe Red Plus Nucleic Acid Dye (10000X) صبغة حمض نووي لامعة باللون الأحمر تم تحسينها وتطويرها على أساس Gelred مع خصائص صبغ الجيل وتم تصميمها لتحل محل عامل التشكيل المرئي شديد السُّمية إثيل بروميد (EB) بأفضل وضوح وحساسية.