5-Aminouridine can modify nucleobases and can be incorporated into the target DNA. 5-Aminouridine exhibits a wide range of biological activity and it inhibits the growth of tumors, fungi and viruses [1] [2].
In Vivo, 5-Aminouridine inhibits incorporation of carbamylaspartate into pyrimidines of both RNA and DNA in rat lived and [32P]phosphate into phospholipids and RNA nucleotides of rat liver slices and hepatoma[1].
In Vitro, The modified nucleobase, 5-Aminouridine, must exhibit a low enough potential to be oxidized by Os(bpy)33+. 5-Aminouridine can be inserted into the target DNA by direct synthesis using modified phosphoramidites[3].
References:
[1]. Smith DA, et al. Studies on 5-aminouridine. Biochim Biophys Acta. 1966 May 19;119(2):221-8.
[2]. ROBERTS M, et al. Antimetabolite activity of uridine and cytidine derivatives. J Biol Chem. 1952 Feb;194(2):695-701. PMID: 14927662....
[3]. MR, et al. Detection of attomole quantities [correction of quantitites] of DNA targets on gold microelectrodes by electrocatalytic nucleobase oxidation. Anal Chem. 2003 Dec 1;75(23):6586-92.
In Vivo, 5-Aminouridine inhibits incorporation of carbamylaspartate into pyrimidines of both RNA and DNA in rat lived and [32P]phosphate into phospholipids and RNA nucleotides of rat liver slices and hepatoma[1].
In Vitro, The modified nucleobase, 5-Aminouridine, must exhibit a low enough potential to be oxidized by Os(bpy)33+. 5-Aminouridine can be inserted into the target DNA by direct synthesis using modified phosphoramidites[3].
References:
[1]. Smith DA, et al. Studies on 5-aminouridine. Biochim Biophys Acta. 1966 May 19;119(2):221-8.
[2]. ROBERTS M, et al. Antimetabolite activity of uridine and cytidine derivatives. J Biol Chem. 1952 Feb;194(2):695-701. PMID: 14927662....
[3]. MR, et al. Detection of attomole quantities [correction of quantitites] of DNA targets on gold microelectrodes by electrocatalytic nucleobase oxidation. Anal Chem. 2003 Dec 1;75(23):6586-92.