APC-Cy5.5 is a tandem dye widely used in flow cytometry. It consists of the energy donor allophycocyanin ( APC ) and the energy acceptor Cy5.5, and operates via the fluorescence resonance energy transfer mechanism (Ex/Em = 650 nm/690 nm) [1].
In Vitro, APC-Cy5.5-conjugated isotype control antibody (0.5 μg/mL; 30 min) binds weakly to mDEC205-expressing CHO cells, and does not bind to hDEC205-expressing CHO cells[1].
References:
[1]. Park CG, et al. PE-Cy5.5 conjugates bind to the cells expressing mouse DEC205/CD205. J Immunol Methods. 2012;384(1-2):184-190.
In Vitro, APC-Cy5.5-conjugated isotype control antibody (0.5 μg/mL; 30 min) binds weakly to mDEC205-expressing CHO cells, and does not bind to hDEC205-expressing CHO cells[1].
References:
[1]. Park CG, et al. PE-Cy5.5 conjugates bind to the cells expressing mouse DEC205/CD205. J Immunol Methods. 2012;384(1-2):184-190.