BGSN3 is a good substrate for the SNAP-tag and H5 enzymes. The activities of SNAP-tag and H5 enzymes on BGSN3 are reasonable (IC50=17.8 and 10 μM, respectively). BGSN3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.
SNAP-tag® is a powerful technology for the labelling of protein/enzymes by using benzyl-guanine (BG) derivatives as substrates[1].
BGSN3 proved to be a good substrate for the direct immobilisation of these tags (SNAP-tag® and its relative thermo stable version, SsOGT-H5) on solid surfaces[1].
References:
[1]. Merlo R, et al. The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate. J Enzyme Inhib Med Chem. 2021;36(1):85-97.