BZiPAR is a fluorescent probe. BZiPAR also is a substrate of trypsin that becomes fluorescent after cleavage by the protease.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Confocal Imaging of Trypsinogen Activation:
1. Incubate the cells according to your normal protocol.
2. Isolate clusters of acinar cells.
3. Incubate acini in extracellular solution containing 100 μM BZiPAR.
4. BZiPAR were excited with a 488-nm laser line; emission was collected in the 508- to 530-nm band for cleaved BZiPAR.
References:
[1]. Mark W Sherwood, et al. Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells. Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5674-9.