C12E8 (Synonyms: Octaethylene Glycol Monododecyl Ether) |
رقم الكتالوجGC43020
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C12E8 (C12E8) هو منظف غير أيوني يمكن استخدامه لاستخراج البروتين الغشائي.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3055-98-9
Sample solution is provided at 25 µL, 10mM.
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C12E8 is a non-ionic surfactant, its full name is surfactant polyoxyethylene glycol, it belongs to the polyoxyethylene ether surfactant [1]. C12E8 regulates the curvature and morphological transition of phospholipid vesicle membrane in a concentration-dependent manner, promoting natural morphological changes at low concentrations and inducing abnormal deformation and ultimately leading to vesicle disintegration at high concentrations [2-3].
In DC3F cells, C12E8 (0.05mg/mL; 45min) decreases the voltage required for irreversible electroporation in planar lipid bilayers [4]. In erythrocytes, large flat invaginations/endovesicles with a compressed central region and a globular periphery were found in sections of erythrocytes treated with C12E8 (44μM; 60 min) [5].
References:
[1]. Ruiz CC, Molina-Bolívar JA. Characterization of mixed non-ionic surfactants n-octyl-β-d-thioglucoside and octaethylene–glycol monododecyl ether: Micellization and microstructure. Journal of colloid and interface science. 2011 Sep 1; 361(1): 178-185.
[2]. Mavcic B, Babnik B, Iglic A, et al. Shape transformation of giant phospholipid vesicles at high concentrations of C12E8. Bioelectrochemistry. 2004 Jun 1; 63(1-2): 183-187.
[3]. Guo Y. Surface interactions between poly (ethylene glycol) and phospholipid bilayers: Effects on aggregation and fusion. State University of New York at Buffalo; 1993.
[4]. Kanduser M, Fosnaric M, Sentjurc M, et al. Effect of surfactant polyoxyethylene glycol (C12E8) on electroporation of cell line DC3F. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2003 Mar 12; 214(1-3): 205-217.
[5]. Bobrowska-Hägerstrand M, Kralj-Iglič V, Iglič A, et al. Torocyte membrane endovesicles induced by octaethyleneglycol dodecylether in human erythrocytes. Biophysical journal. 1999 Dec 1; 77(6): 3356-3362.
| Cell experiment [1]: | |
Cell lines | DC3F cells |
Preparation Method | The cells in suspension were incubated for 45min at 37℃; 0.05mg/mL C12E8 was added to treated cells, while the medium in which C12E8 was dissolved was added to untreated cells. The membrane fluidity was measured by electron paramagnetic resonance method (EPR), using the spin probe methylester of 5-doxyl palmitate (MeFASL), which is lipophilic and therefore incorporates primarily into the membrane lipid bilayer. Sixty milliliters of 0.1mM MeFASL in ethanol solution was placed into the glass tube, then ethanol was evaporated by rotavapor. 1mL of cell suspension that contained 20 × 106 cells was added to the MeFASLfilm formed on the wall of the glass tube, and incubated for 15min while shaking. After that, the cell suspension was centrifuged and the pellet was placed in glass capillary for EPR measurements. From the EPR spectra at 4℃, the maximal hyperfine splitting constant 2AII that reflects the order parameter was measured. |
Reaction Conditions | 0.05mg/mL; 45min |
Applications | C12E8 decreases the voltage required for irreversible electroporation in planar lipid bilayers. |
References: | |
| Cas No. | 3055-98-9 | SDF | |
| المرادفات | Octaethylene Glycol Monododecyl Ether | ||
| Canonical SMILES | CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCO | ||
| Formula | C28H58O9 | M.Wt | 538.8 |
| الذوبان | DMF: 30 mg/ml,DMSO: 15 mg/ml,Ethanol: 30 mg/ml,PBS (pH 7.2): 1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.856 mL | 9.2799 mL | 18.5598 mL |
| 5 mM | 371.2 μL | 1.856 mL | 3.712 mL |
| 10 mM | 185.6 μL | 928 μL | 1.856 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: An oil
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)