Copper Fluor-4 (Synonyms: CF4) |
رقم الكتالوجGC73290
One-Click Copy Product Info
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Copper Fluor-4 (CF4) هو مسبار الفلورسنت الخاص بالنحاس + على أساس ناقلة صبغة رودول.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2365532-64-3
Sample solution is provided at 25 µL, 10mM.
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Copper Fluor-4 (CF4) is a Cu+-specific fluorescent probe based on a rhodol dye scaffold. Copper Fluor-4 has high copper selectivity with a Kd value of 2.9×10−13 M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper Fluor-4 is stable in a psiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission).
Copper Fluor-4 (0.8 µM, 10 min) demonstrates localization of the copper in the soma region, which includes its localization in the perinuclear Golgi region in the PC-12-derived neurons[3].
Copper Fluor-4 (2 µM, 24 h) can be used to assess changes in labile copper distributions in small living organisms such as zebrafish embryos[1].Copper Fluor-4 (10 µM, 3 h) can be used to assess labile Cu levels with a Kd value of 2.9×10−13 M for a 1:1 copper:probe stoichiometry that is well-matched to monitor labile Cu pools by reversible Cu binding without depleting the total Cu store in worms[2].
References:
[1]. Xiao T, et al. Copper regulates rest-activity cycles through the locus coeruleus-norepinephrine system. Nat Chem Biol. 2018 Jul;14(7):655-663.
[2]. Weishaupt AK, et al. Dysfunction in atox-1 and ceruloplasmin alters labile Cu levels and consequently Cu homeostasis in C. elegans. Front Mol Biosci. 2024 Feb 8;11:1354627.
[3]. Chakraborty K, et al. Copper dependent ERK1/2 phosphorylation is essential for the viability of neurons and not glia. Metallomics. 2022 Apr 1;14(4):mfac005.
| Cas No. | 2365532-64-3 | SDF | |
| المرادفات | CF4 | ||
| Formula | C38H47F3N2O2S4 | M.Wt | 749.05 |
| الذوبان | DMSO : 125 mg/mL (166.88 mM; Need ultrasonic) | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.335 mL | 6.6751 mL | 13.3502 mL |
| 5 mM | 267 μL | 1.335 mL | 2.67 mL |
| 10 mM | 133.5 μL | 667.5 μL | 1.335 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: An oil
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)