Ethylmalonyl-CoA pentalithium (Ethylmalonyl coenzyme A pentalithium) is a metabolic intermediate and biosynthetic precursor associated with crotonyl-CoA carboxylase/reductase. Ethylmalonyl‑CoA pentalithium is generated via the reductive carboxylation of crotonyl-CoA catalyzed by crotonyl-CoA carboxylase/reductase ( CCR ); it can be recognized by the PKS acyltransferase AT and participate in the biosynthesis of antibiotic polyketide natural products. Ethylmalonyl-CoA pentalithium is used in biosynthesis-related research [1] [2].
In Vitro, Ethylmalonyl‑CoA pentalithium can be produced via the reductive carboxylation of acetyl-CoA-derived intermediates in cell extracts of Rhodobacter sphaeroides cultured with acetate as the aerobic substrate; it can also be catalyzed to undergo reverse decomposition by purified recombinant Rhodobacter sphaeroides crotonyl-CoA carboxylase/reductase[1][2]. Ethylmalonyl‑CoA pentalithium serves as a key precursor for the biosynthesis of polyketide natural products including monensin A, sanglifehrin A, davilactone, and bacillomycin [2].
References:
[1]. Erb TJ, et al. Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway. Proceedings of the National Academy of Sciences of the United States of America. 2007 Jun 19;104(25):10631-6.
[2]. Wilson MC, et al. Beyond ethylmalonyl-CoA: the functional role of crotonyl-CoA carboxylase/reductase homologs in expanding polyketide diversity. Natural product reports. 2012 Jan;29(1):72-86.
In Vitro, Ethylmalonyl‑CoA pentalithium can be produced via the reductive carboxylation of acetyl-CoA-derived intermediates in cell extracts of Rhodobacter sphaeroides cultured with acetate as the aerobic substrate; it can also be catalyzed to undergo reverse decomposition by purified recombinant Rhodobacter sphaeroides crotonyl-CoA carboxylase/reductase[1][2]. Ethylmalonyl‑CoA pentalithium serves as a key precursor for the biosynthesis of polyketide natural products including monensin A, sanglifehrin A, davilactone, and bacillomycin [2].
References:
[1]. Erb TJ, et al. Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway. Proceedings of the National Academy of Sciences of the United States of America. 2007 Jun 19;104(25):10631-6.
[2]. Wilson MC, et al. Beyond ethylmalonyl-CoA: the functional role of crotonyl-CoA carboxylase/reductase homologs in expanding polyketide diversity. Natural product reports. 2012 Jan;29(1):72-86.