Fluorescein-NAD+ sodium

Fluorescein-NAD + sodium is a fluorescent NAD + analog that can be used in studies related to the detection of ADP-ribosylation reactions. Fluorescein-NAD + sodium enables visualization of ADP-ribose groups, thereby facilitating the detection of ADP-ribosylation reactions. Fluorescein-NAD + sodium serves as a substrate for detecting the auto-ribosylation activity of SIRT6 and the ADP-ribosylation of RhoA by C3larvin toxin ^{[1] [2]}.

In Vitro, Fluorescein-NAD+ sodium detects the enhanced mono-ADP-ribosylation activity of SIRT6 induced by Fucoidan-FV^[1]. Fluorescein-NAD+ sodium can be used to detect the ADP-ribosylation modification of human GST-RhoA (ΔCAAX) by C3larvin toxin, confirming that RhoA is its substrate; it also enables quantitative analysis of C3larvin-mediated ADP-ribosylation of human GST-RhoA (ΔCAAX) via fluorescence intensity measurement^[2]. Fluorescein-NAD+ sodium enables the detection of the NAD+-targeted ADP-ribosyltransferase activity of C3larvin using human GST-RhoA (ΔCAAX), which has a Km value of 33.6 μM^[2].

References:
[1]. Robbins P, et al. Fucoidans are senotherapeutics that enhance SIRT6‑dependent DNA repair. Research Square, 2025.
[2]. Krska D, et al. C3larvin toxin, an ADP-ribosyltransferase from Paenibacillus larvae. The Journal of biological chemistry. 2015 Jan 16;290(3):1639-53.