GLPBio

Vigabatrin

Catalog No.: GC37905Purity:>98.00%Synonyms: γ-Vinyl GABA
COASDSData Sheet
Vigabatrin (γ-Vinyl-GABA) ، وهو ناقل عصبي مثبط مشتق من الفينيل GABA ، هو مثبط ترانس أميناز GABA النشط عن طريق الفم ولا رجوع فيه

المنتجات مخصصة للاستخدام البحثي فقط، وليست للاستخدام البشري. نحن لا نبيع للمرضى.

Vigabatrin
Cas No.: 68506-86-5
10mM (in 1mL Water)In Stock
$62.00
1
5mgIn Stock
$35.00
1
10mgIn Stock
$56.00
1
50mgIn Stock
$224.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

المراجع (Citations)

لا تتوفر حاليًا مراجع لهذا المنتج. فيما يلي مجموعة مختارة من المنشورات التي تستشهد بمنتجات GlpBio.
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    641, 529–536 (2025)
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    632, 686–694 (2024)
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    618, 1017–1023 (2023)
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    Nature
    610, 366–372 (2022)
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    Cell
    187(9):2288-2304 (2024)
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    Cell
    183(7):1867-1883 (2020)
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    Science
    388(6745) (2025)
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الوصف (Description)

Vigabatrin is an irreversible inhibitor of γ-aminobutyric acid transaminase (GABA-T), with potent activity in elevating brain GABA levels and enhancing inhibitory neurotransmission [1-3]. Vigabatrin is commonly used to treat refractory epilepsy [4].

In glioblastoma multiforme cell (13–06-MG), concentration-dependent inhibitory effect of Vigabatrin (0.3-100μM; 24h) on the activity of IKCa channels [5]. In Caco-2 and MDCK cells, Vigabatrin (30mM; 24h) inhibits the uptake of taurine in Caco-2 and MDCK cells to 34 ± 3 and 53 ± 2% [6]. In U87 cells, Vigabatrin administration has a growth-promoting effect on cells [7].

In Tsc1GFAPCKO mice, Vigabatrin (50mg/kg, 100mg/kg, 200mg/kg; ip; 10 weeks) treatment decreased the development of seizures and modestly improved survival [8]. In C57BL/6J mice, after of Vigabatrin (140mg/kg; ip; 4 weeks) treatment, ERG b-wave amplitude was enhanced, and amplitudes of oscillatory potentials were reduced [9]. In C57BL/6J mice, Vigabatrin (35-140mg/kg; sc; 14d) treatment induces amino acid disturbances in mice [10].

References:
[1]. French JA. Vigabatrin. Epilepsia. 1999 May; 40: s11-6.
[2]. James Willmore L, Abelson MB, Ben‐Menachem E, et al. Vigabatrin: 2008 update. Epilepsia. 2009 Feb; 50(2): 163-173.
[3]. Ben‐Menachem E. Mechanism of action of vigabatrin: correcting misperceptions. Acta neurologica scandinavica. 2011 Dec; 124: 5-15.
[4]. Tartara A, Manni R, Galimberti CA, et al. Vigabatrin in the treatment of epilepsy: a double‐blind, placebo‐controlled study. Epilepsia. 1986 Dec; 27(6): 717-723.
[5]. Hung TY, Huang HY, Wu SN, et al. Depressive effectiveness of vigabatrin (γ-vinyl-GABA), an antiepileptic drug, in intermediate-conductance calcium-activated potassium channels in human glioma cells. BMC Pharmacology and Toxicology. 2021 Dec; 22: 1-9.
[6]. Plum J, Nøhr MK, Hansen SH, et al. The anti-epileptic drug substance vigabatrin inhibits taurine transport in intestinal and renal cell culture models. International journal of pharmaceutics. 2014 Oct 1; 473(1-2): 395-397.
[7]. Lee CY, Lai HY, Chiu A, et al. The effects of antiepileptic drugs on the growth of glioblastoma cell lines. Journal of neuro-oncology. 2016 May; 127: 445-453.
[8]. Zhang B, McDaniel SS, Rensing NR, et al. Vigabatrin inhibits seizures and mTOR pathway activation in a mouse model of tuberous sclerosis complex. PLoS One. 2013 Feb 20; 8(2): e57445.
[9]. Chan K, Hoon M, Pattnaik BR, et al. Vigabatrin-induced retinal functional alterations and second-order neuron plasticity in C57BL/6J mice. Investigative Ophthalmology & Visual Science. 2020 Feb 7; 61(2): 17.
[10]. Walters DC, Arning E, Bottiglieri T, et al. Metabolomic analyses of vigabatrin (VGB)-treated mice: GABA-transaminase inhibition significantly alters amino acid profiles in murine neural and non-neural tissues. Neurochemistry international. 2019 May 1; 125: 151-162.

البروتوكول (Protocol)

Cell experiment [1]:

Cell lines

Glioblastoma multiforme cell (13–06-MG)

Preparation Method

The glioblastoma multiforme cell (13–06-MG) were cultured at a density of 106/mL in high glucose (4g/L) Dulbecco’s modified Eagle media supplemented with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin and 10μg/mL streptomycin. Cells were maintained at 37 ℃ in a 5% CO2 incubator as monolayer cultures and thereafter sub-cultured weekly. Fresh media was added every 2–3 days in order to ensure a healthy cell population. To evaluate concentration-dependent inhibition of VGB on the probability of IKCa channels that would be open, we kept 13–06-MG cells to be bathed in normal Tyrode’s solution containing 1.8mM CaCl2, and each cell examined was voltage-clamped at -80mV relative to the bath. The probability of channel opening was measured in the control or during cell exposure to different concentrations (0.3-100μM) of Vigabatrin; and, these values were then compared with those taken after the addition of TRAM-34 (3μM). TRAM-34 is a known selective blocker of IKCa channels.

Reaction Conditions

0.3-100μM; 24h

Applications

Concentration-dependent inhibitory effect of Vigabatrin on the activity of IKCa channels.

Animal experiment [2]:

Animal models

Tsc1GFAPCKO mice

Preparation Method

Three-week-old Tsc1GFAPCKO mice were treated with vehicle (saline) or Vigabatrin at different doses (50, 100, 200mg/kg/day, i.p) for one week for Western blot and GABA concentrations, for four weeks (200mg/kg/day) for histology and immunohistochemistry analysis, and for up to 10 weeks (200mg/kg/day) for video-EEG monitoring.

Dosage form

50mg/kg, 100mg/kg, 200mg/kg; ip; 10 weeks

Applications

Vigabatrin treatment decreased the development of seizures and modestly improved survival in Tsc1GFAPCKO mice.

References:
[1]. Hung TY, Huang HY, Wu SN, et al. Depressive effectiveness of vigabatrin (?-vinyl-GABA), an antiepileptic drug, in intermediate-conductance calcium-activated potassium channels in human glioma cells. BMC Pharmacology and Toxicology. 2021 Dec; 22: 1-9.
[2]. Zhang B, McDaniel SS, Rensing NR, et al. Vigabatrin inhibits seizures and mTOR pathway activation in a mouse model of tuberous sclerosis complex. PLoS One. 2013 Feb 20; 8(2): e57445.

وثائق المنتج (Product Documents)

Purity:>98.00%Appearance:A solid
COASDSData Sheet

الخصائص الكيميائية (Chemical Properties)

رقم CAS
68506-86-5
المرادفات
γ-Vinyl GABA
الاسم الكيميائي
4-amino-5-hexenoic acid
SMILES
C=CC(N)CCC(O)=O
الصيغة الجزيئية
C6H11NO2
الوزن الجزيئي
129.16
الذوبانية
10mg/mL in PBS, pH 7.2
التخزين
Store at -20°C
إرشادات عامة
اختر مذيبًا مناسبًا وفقًا لبيانات ذوبانية المنتج. بعد التحضير، قسّم المحلول المخزون إلى أجزاء لتجنب دورات التجميد والذوبان المتكررة.
استخدم المحاليل المخزونة خلال 6 أشهر عند حفظها في -80°C، أو خلال شهر واحد عند حفظها في -20°C.
لتحسين الذوبانية، سخّن الأنبوب إلى 37°C وعرّضه للموجات فوق الصوتية لمدة مناسبة.
شروط الشحن
تُشحن عينات التقييم مع عبوات تبريد. وتُشحن الأحجام الأخرى المتاحة في درجة حرارة الغرفة، أو مع عبوات تبريد عند الطلب.

الحاسبات (Calculators)المولارية / التخفيف / الوزن الجزيئي / تحويل الوحدات / تركيبة داخل الجسم الحي / الذوبانية

g/mol

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