Clodronate liposomes, a mixture of Clodronate encapsulated by phospholipids, is a macrophage scavenger. Clodronate liposomes is engulfed by macrophages in the body. Under the action of lysosomal phosphatases in macrophages, Clodronate dissolved in liposomes will be gradually released and accumulated in cells. When a certain concentration is reached, macrophages will be irreversibly damaged and apoptosis will be induced [1].
In Vivo, Methods for clearing macrophages from different tissues (for reference only, can be optimized according to specific experiments) Clearing red pulp area of spleen and Kupffer macrophages: Single dose: 200 μL/mouse (intravenous or intraperitoneal injection). Long-term administration: The first dose is 150 μL/mouse, and then 100 μL/mouse every three days. Depletion of alveolar macrophages The best effect was obtained by intravenous injection (150-200 μL) combined with tracheal or intranasal administration (50 μL). Depletion of brain microglia Intraventricular cerebrospinal fluid injection, mice (10 μL), rats (50 μL).
References:
[1]. Moreno SG. Depleting Macrophages In Vivo with Clodronate-Liposomes. Methods Mol Biol. 2018;1784:259-262.
In Vivo, Methods for clearing macrophages from different tissues (for reference only, can be optimized according to specific experiments) Clearing red pulp area of spleen and Kupffer macrophages: Single dose: 200 μL/mouse (intravenous or intraperitoneal injection). Long-term administration: The first dose is 150 μL/mouse, and then 100 μL/mouse every three days. Depletion of alveolar macrophages The best effect was obtained by intravenous injection (150-200 μL) combined with tracheal or intranasal administration (50 μL). Depletion of brain microglia Intraventricular cerebrospinal fluid injection, mice (10 μL), rats (50 μL).
References:
[1]. Moreno SG. Depleting Macrophages In Vivo with Clodronate-Liposomes. Methods Mol Biol. 2018;1784:259-262.