MOTS-c |
رقم الكتالوجGC25648
One-Click Copy Product Info
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MOTS-c is a mitochondrial-derived polypeptide with metabolic regulatory functions.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1627580-64-6
Sample solution is provided at 25 µL, 10mM.
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MOTS-c is a mitochondrial-derived polypeptide with metabolic regulatory functions [1]. MOTS-c exerts anti-injury and anti-inflammatory effects by activating the AMPK pathway and inhibiting the MAP kinase-c-fos signaling pathway [2]. MOTS-c can be used in studies on stress, inflammation, metabolism, neuroprotection, and aging [3].
In vitro, MOTS-c (0-100μM; 8-24 hours) can promote the phosphorylation of AMPK (Thr 172) and Akt (Ser 473) in HEK 293 cells in a time- and dose-dependent manner [4]. Different concentrations of MOTS-c (0, 2.5, 5, 10, 20 and 40μM; 24 hours) would weaken the inhibitory effect of hypoxia treatment on the inhibition of DR4, DR5, FADD and caspase-8 in HCC cells. MOTS-c weakens the resistance of liver cancer cells to TRAIL-induced apoptosis by activating MEF2A [5].
In vivo, male C57 BL/6 mice treated with MOTS-c (5mg/kg/day; i.p.) for 7 days significantly enhanced insulin-stimulated glucose clearance rate and improved insulin sensitivity in skeletal muscle [4]. Treating mice with MOTS-c (50mg/kg; i.p.) 4 hours before formalin treatment significantly reduced the levels of pro-inflammatory cytokines in mouse serum and increased the levels of anti-inflammatory cytokines. Additionally, MOTS-c treatment significantly increased the phosphorylation level of AMPKα, inhibited the activation of spinal extracellular signal-regulated kinase (ERK), c-Jun amino-terminal kinase (JNK), and P38 in mice induced by formalin, as well as the expression of c-fos [6].
References:
[1] Benayoun B A, Lee C. MOTS‐c: a mitochondrial‐encoded regulator of the nucleus[J]. Bioessays, 2019, 41(9): 1900046.
[2] Yin X, Jing Y, Chen Q, Abbas AB, Hu J, Xu H. The intraperitoneal administration of MOTS-c produces antinociceptive and anti-inflammatory effects through the activation of AMPK pathway in the mouse formalin test. Eur J Pharmacol. 2020 Mar 5;870:172909.
[3] Wan W, Zhang L, Lin Y, et al. Mitochondria-derived peptide MOTS-c: effects and mechanisms related to stress, metabolism and aging[J]. Journal of Translational Medicine, 2023, 21(1): 36.
[4] Lee C, Zeng J, Drew BG, Sallam T, Martin-Montalvo A, Wan J, Kim SJ, Mehta H, Hevener AL, de Cabo R, Cohen P. The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance. Cell Metab. 2015 Mar 3;21(3):443-54.
[5] Shen H, Nie J, Wang X, et al. MOTS-c relieves hepatocellular carcinoma resistance to TRAIL-induced apoptosis under hypoxic conditions by activating MEF2A[J]. Experimental Cell Research, 2025, 444(1): 114354.
[6] Yin X, Jing Y, Chen Q, et al. The intraperitoneal administration of MOTS-c produces antinociceptive and anti-inflammatory effects through the activation of AMPK pathway in the mouse formalin test[J]. European journal of pharmacology, 2020, 870: 172909.
| Cell experiment [1]: | |
Cell lines | HEK293 cells |
Preparation Method | HEK293 cells are usually cultured in the Dulbecco-modified Eagle's Medium (DMEM). Since the main function of mitochondria involves metabolic processes, the next step is to investigate the impact of MOTS-c on cell metabolism using an unbiased global metabolomics analysis method in vitro. Using a "function-enhancing" model, studies were conducted using HEK293 cells with stable overexpression of MOTS-c (MOTS-c-ST) or with 10μM synthetic MOTS-c for 24 hours and 72 hours of exogenous treatment. Microarray analysis was performed on HEK293 cells treated with MOTS-c for 4 hours and 72 hours. The level of AICAR measured in MOTS-c-ST cells by mass spectrometry. |
Reaction Conditions | 10μM; 4h, 72h |
Applications | MOTS-c targets the methionine-folate cycle, increases the level of AICAR, and activates AMPK. |
| Animal experiment [1]: | |
Animal models | ICR mice |
Preparation Method | In all in vivo experiments, MOTS-c was injected intraperitoneally daily. Eight-week-old CD-1 (ICR) mice were purchased from Harlan Company. C57BL/6 mice were purchased from Jackson Laboratory. The mice were fed a high-fat diet (containing 60% calories) and a matched control diet for 8 weeks. The diet was changed twice a week, and body weight and food intake were recorded daily (N=10). Seven days after intraperitoneal injection of MOTS-c, a glucose tolerance test (GTT) was conducted. Subsequently, a hyperinsulinemic-euglycemic clamp test was performed to quantify the effect of 7 days of MOTS-c treatment on systemic insulin sensitivity. |
Dosage form | 5mg/kg/day for 7 days; i.p. |
Applications | MOTS-c significantly enhanced the glucose clearance rate stimulated by insulin and improved insulin sensitivity in skeletal muscle. |
References: | |
| Cas No. | 1627580-64-6 | SDF | |
| Formula | C101H152N28O22S2 | M.Wt | 2174.6 |
| الذوبان | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 459.9 μL | 2.2993 mL | 4.5985 mL |
| 5 mM | 92 μL | 459.9 μL | 919.7 μL |
| 10 mM | 46 μL | 229.9 μL | 459.9 μL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
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Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)