Phytosphingosine-d7 is intended for use as an internal standard for the quantification of phytosphingosine by GC- or LC-MS. Phytosphingosine is a sphingolipid with a hydroxyl group at the C4 position that is found mainly in fungi and plants but also in animals, including humans.[1],[2] It is metabolized to odd-numbered fatty acids with 2-hydroxy palmitic acid as an intermediate.[3] Phytosphingosine dose-dependently induces cell death of CHO cells and inhibits carbachol-induced activation of phospholipase D (PLD) in CHO cells transfected with C. elegans muscarinic acetylcholine receptors (mAChRs).[4] It is essential in the heat stress response in S. cerevisiae.[5]
References:
[1].Dickson, R.C.Sphingolipid functions in Saccharomyces cerevisiae: Comparison to mammalsAnnu. Rev. Biochem.6727-48(1998).
[2].Schürer, N.Y., Plewig, G., and Elias, P.M.Stratum corneum lipid functionDermatologica183(2)77-94(1991).
[3].Kondo, N., Ohno, Y., Yamagata, M., et al.Identification of the phytosphingosine metabolic pathway leading to odd-numbered fatty acidsNat. Commun.55338(2014).
[4].Lee, J.S., Min, D.S., Park, C., et al.Phytosphingosine and C2-phytoceramide induce cell death and inhibit carbachol-stimulated phospholipase D activation in Chinese hamster ovary cells expressing the Caenorhabditis elegans muscarinic acetylcholine receptorFEBS. Lett.499(1-2)82-86(2001).
[5].Hannun, Y.A., Luberto, C., and Argraves, K.M.Enzymes of sphingolipid metabolism: From modular to integrative signalingBiochemistry40(16)4893-4903(2001).