Sphingosine-1-phosphate
Catalog No.: GC17004Purity:>98.00%Synonyms: Sphingosine-1-phosphate (d18:1); S1P (d18:1); Sphingosine-1-Phosphoric Acid
فوسفات السفينجوزين 1 هو منتج ميتابولي من سفينجوليبيدات غشاء الخلية.
المنتجات مخصصة للاستخدام البحثي فقط، وليست للاستخدام البشري. نحن لا نبيع للمرضى.
Cas No.: 26993-30-6
1mgIn Stock
$131.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 1mg | $131.00 | In Stock | 1 |
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المراجع (Citations)
2 publicationsمنشورات الباحثين الذين استخدموا هذا المنتجالوصف (Description)
Sphingosine-1-phosphate, a lipid mediator formed by the metabolism of sphingomyelin, is an agonist of S1P receptors[1]. Sphingosine-1-phosphate is secreted into the extracellular environment and signals via G protein-coupled S1P receptors to regulate cell-cell and cell-matrix adhesion, and thereby influence cell migration, differentiation, and survival[2]. Sphingosine-1-phosphate activates the ERK signaling pathway to stimulate DNA synthesis, reduces endothelial permeability, and induces cortical reorganization of the endothelial cell cytoskeleton by regulating the Rho and Rac signaling pathways[3]. Sphingosine-1-phosphate has been widely used in signal cascade studies to modulate vascular barrier function and immune function[4].
In vitro, Sphingosine-1-phosphate (1µM) was pre-treated for 1 hour, which significantly prevented the HK-2 cell death induced by ischemia/reperfusion (I/R) and activated the JAK2/STAT3 pathway[5]. Treatment with 400µM Sphingosine-1-phosphate for 4 days markedly suppressed cell apoptosis in the ovarian cortical tissues and reduced the expression of cleaved caspase-3[6]. Treatment with sphingosine-1-phosphate (1µM) for 19 hours significantly reduced cell death in SH-SY5Y cells under oxygen-glucose deprivation (OGD) conditions, decreased OGD-induced membrane depolarization, and promoted the translocation of PKCε to the mitochondria[7].
In vivo, Sphingosine-1-phosphate treatment via subcutaneous injection at a dose of 10ng/day 7 days increased the cholinergic reactivity of bronchial tissues in BALB/c mice[8]. A single intraperitoneal injection of Sphingosine-1-phosphate (100μg/kg) alleviated lung injury induced by intestinal I/R in mice, reducing the levels of pro-inflammatory cytokines in plasma[9].
References:
[1] Maceyka M, Harikumar K B, Milstien S, et al. Sphingosine-1-phosphate signaling and its role in disease[J]. Trends in cell biology, 2012, 22(1): 50-60.
[2] Mendelson K, Evans T, Hla T. Sphingosine 1-phosphate signalling[J]. Development, 2014, 141(1): 5-9.
[3] Hait N C, Oskeritzian C A, Paugh S W, et al. Sphingosine kinases, sphingosine 1-phosphate, apoptosis and diseases[J]. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2006, 1758(12): 2016-2026.
[4] Blaho V A, Hla T. An update on the biology of sphingosine 1-phosphate receptors[J]. Journal of lipid research, 2014, 55(8): 1596-1608.
[5] de Assis J L, Grelle G M R S, Fernandes A M, et al. Sphingosine 1-phosphate protective effect on human proximal tubule cells submitted to an in vitro ischemia model: the role of JAK2/STAT3: de Assis et al[J]. Journal of physiology and biochemistry, 2024, 80(4): 831-843.
[6] Guzel Y, Bildik G, Oktem O. Sphingosine-1-phosphate protects human ovarian follicles from apoptosis in vitro[J]. European Journal of Obstetrics & Gynecology and Reproductive Biology, 2018, 222: 19-24.
[7] Agudo-López A, Miguel B G, Fernández I, et al. Involvement of mitochondria on neuroprotective effect of sphingosine-1-phosphate in cell death in an in vitro model of brain ischemia[J]. Neuroscience letters, 2010, 470(2): 130-133.
[8] Roviezzo F, D'Agostino B, Brancaleone V, et al. Systemic administration of sphingosine-1-phosphate increases bronchial hyperresponsiveness in the mouse[J]. American journal of respiratory cell and molecular biology, 2010, 42(5): 572-577.
[9] Ding R, Han J, Tian Y, et al. Sphingosine-1-phosphate attenuates lung injury induced by intestinal ischemia/reperfusion in mice: role of inducible nitric-oxide synthase[J]. Inflammation, 2012, 35(1): 158-166.
البروتوكول (Protocol)
| Cell experiment [1]: | |
|
Cell lines |
SH-SY5Y cells |
|
Preparation Method |
SH-SY5Y cells were cultured in DMEM medium supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, and 1% glutamine in a humidified incubator containing 5% CO2 at 37°C. Cells were exposed to OGD for 3h, followed by 16h of recovery, with or without Sphingosine-1-phosphate (0, 0.1, 0.5, and 1μM), and cell viability was measured. |
|
Reaction Conditions |
0, 0.1, 0.5, and 1µM; 19h |
|
Applications |
Sphingosine-1-phosphate treatment enhanced the cell viability of SH-SY5Y cells under OGD conditions in a dose-dependent manner. |
| Animal experiment [2]: | |
|
Animal models |
Male C57BL/6 mice |
|
Preparation Method |
Male C57BL/6 mice (8-week-old) were maintained in a controlled environment at 22-24°C and 60% relative humidity under a 12h light/dark cycle, and were given free access to food and water. Mice were fasted overnight before the experiment but had free access to water. Mice were anesthetized with 3% isoflurane and the abdomen was shaved and rinsed with 70% isopropanol solution. Anesthesia was maintained using 1% isoflurane and body temperature was maintained at 37°C using a heating pad. A midline laparotomy was made and, using a dissecting microscope, the superior mesenteric artery (SMA) as well as the collateral branch from the celiac axis were identified and occluded with nontraumatic vascular clamps. After 45 min of ischemia, the SMA was re-perfused. Then the abdomen was closed. Ischemia was determined by the existence of pulseless or pale color of the small intestine. The return of pulse and restoration of pink color were assumed to be due to the reperfusion of the intestine. In the sham-operated group, the SMA and collateral branches from the celiac axis were identified but were not occluded. Mice were randomized to three groups: (1) sham-operated mice (sham group; n=8), (2) mice exposed to SMA occlusion for 45min followed by reperfusion for 6h (I/R group; n=8), or (3) mice exposed to I/R that received Sphingosine-1-phosphate (I/R+S1P group; n=8). As soon as the abdomen was closed, Sphingosine-1-phosphate (100μg/kg; dissolved in 0.9% saline solution) was administered by intraperitoneal injection. An equal volume of saline was administered to the sham group and I/R group as a vehicle. After 6-h reperfusion, the lung tissues of the mice were collected for analysis. |
|
Dosage form |
100μg/kg; once; i.p. |
|
Applications |
Sphingosine-1-phosphate markedly attenuated I/R-induced lung injury, manifested by the improvement of histological changes and significant decreases of lung water content. |
References: [1] Agudo-López A, Miguel B G, Fernández I, et al. Involvement of mitochondria on neuroprotective effect of sphingosine-1-phosphate in cell death in an in vitro model of brain ischemia[J]. Neuroscience letters, 2010, 470(2): 130-133. [2] Ding R, Han J, Tian Y, et al. Sphingosine-1-phosphate attenuates lung injury induced by intestinal ischemia/reperfusion in mice: role of inducible nitric-oxide synthase[J]. Inflammation, 2012, 35(1): 158-166. | |
وثائق المنتج (Product Documents)
Purity:>98.00%Appearance:A solid
البيانات البيولوجية (Biological Data)
1 / 2
الخصائص الكيميائية (Chemical Properties)
رقم CAS
26993-30-6
المرادفات
Sphingosine-1-phosphate (d18:1); S1P (d18:1); Sphingosine-1-Phosphoric Acid
الاسم الكيميائي
(2S,3R,E)-2-amino-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate
SMILES
O[C@H](/C=C/CCCCCCCCCCCCC)[C@H](COP(O)(O)=O)N
الصيغة الجزيئية
C18H38NO5P
الوزن الجزيئي
379.5
الذوبانية
0.3M NaOH: 4 mg/ml,DMF: <50 µg/ml,DMSO: <50 µg/ml,PBS pH 7.2: <50 µg/ml
التخزين
Store at -20°C
إرشادات عامة
اختر مذيبًا مناسبًا وفقًا لبيانات ذوبانية المنتج. بعد التحضير، قسّم المحلول المخزون إلى أجزاء لتجنب دورات التجميد والذوبان المتكررة.
استخدم المحاليل المخزونة خلال 6 أشهر عند حفظها في -80°C، أو خلال شهر واحد عند حفظها في -20°C.
لتحسين الذوبانية، سخّن الأنبوب إلى 37°C وعرّضه للموجات فوق الصوتية لمدة مناسبة.
استخدم المحاليل المخزونة خلال 6 أشهر عند حفظها في -80°C، أو خلال شهر واحد عند حفظها في -20°C.
لتحسين الذوبانية، سخّن الأنبوب إلى 37°C وعرّضه للموجات فوق الصوتية لمدة مناسبة.
شروط الشحن
تُشحن عينات التقييم مع عبوات تبريد. وتُشحن الأحجام الأخرى المتاحة في درجة حرارة الغرفة، أو مع عبوات تبريد عند الطلب.
الحاسبات (Calculators)المولارية / التخفيف / الوزن الجزيئي / تحويل الوحدات / تركيبة داخل الجسم الحي / الذوبانية
g/mol
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