Trypaflavin bromide

Trypaflavin bromide is an orally active acridine compound and antimalarial agent. Trypaflavin bromide invades germ cells. Trypaflavin bromide induces aberrations in unfertilized oocytes. Trypaflavin bromide increases the frequency of chromosomal aberrations. Trypaflavin bromide shows weak mutagenicity. Trypaflavin bromide is highly toxic to Leishmania, causing immediate lysis of the leptomonads ^{[1] [2]}.

In Vivo, Trypaflavin (50 mg/kg; p.o.; single acute dose; day 7 post conception) bromide induces dominant lethal mutations in F1 female offspring of pregnant C3H mice, with an induced mutation frequency of 13.25^[1]. Trypaflavin (2 mg/kg/day; p.o.; 50 days) bromide slightly elevates preimplantation egg loss and dead implants without inducing significant dominant lethal mutations in female C3H mice, and weakly increases total chromosome aberration frequencies to 21.1% in metaphase-II oocytes of female NMRI mice^[1].

References:
[1]. Basler A, et al. Comparative investigations with trypaflavin in metaphase-II oocytes and in dominant lethal assay. Hum Genet. 1977;40(1):87-92.
[2]. Senekji H A. The effect of sulfanilamide and trypaflavin on cultures of Leishmania tropica. Journal of Infectious Diseases, 1940, 66(2): 111-112.