Vari Fluor 405-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=405 nm/431 nm. Product Concentration: 0.8 mg/mL
In Vitro, General Protocol 1.Protein treatment Before use, centrifuge at 5000× g for 3 min, only the supernatant is used for experiments to eliminate protein aggregates and reduce non-specific background staining. 2.Labeling Dilute Vari Fluor-Streptavidin at 1:500-1:1000, which can be adjusted according to the specific conditions of the protein or antibody. Storage -20°C, Protect from light Precautions 1. The actual content of the dye is small, dissolve it directly in the tube after receiving it for experiments. 2. Before use, please centrifuge the product to the bottom of the tube instantaneously before subsequent experiments. 3. This product is intended for scientific research only by professionals and is not to be used for clinical diagnosis or treatment, food or medicine. 4. For your safety and health, please wear lab coat and disposable gloves.
References:
[1]. Nanda JS, et al. Labeling a protein with fluorophores using NHS ester derivitization. Methods Enzymol. 2014;536:87-94.
In Vitro, General Protocol 1.Protein treatment Before use, centrifuge at 5000× g for 3 min, only the supernatant is used for experiments to eliminate protein aggregates and reduce non-specific background staining. 2.Labeling Dilute Vari Fluor-Streptavidin at 1:500-1:1000, which can be adjusted according to the specific conditions of the protein or antibody. Storage -20°C, Protect from light Precautions 1. The actual content of the dye is small, dissolve it directly in the tube after receiving it for experiments. 2. Before use, please centrifuge the product to the bottom of the tube instantaneously before subsequent experiments. 3. This product is intended for scientific research only by professionals and is not to be used for clinical diagnosis or treatment, food or medicine. 4. For your safety and health, please wear lab coat and disposable gloves.
References:
[1]. Nanda JS, et al. Labeling a protein with fluorophores using NHS ester derivitization. Methods Enzymol. 2014;536:87-94.