XL44, an hRpn13 binder, induces hRpn13-dependent apoptosis and also restricts cell viability by a PCLAF-dependent mechanism. XL44 induces ubiquitin-dependent loss of hRpn13Pru and ubiquitin-independent loss of select KEN box containing proteins.
XL44 (20 μM, 4-24 h, lysates from RPMI 8226 cells) induces apoptosis in an hRpn13-dependent manner and reduces hRpn13Pru levels[1].
References:
[1]. Xiuxiu Lu, et al. A structure-based designed small molecule depletes hRpn13Pru and a select group of KEN box proteins. Nat Commun. 2024 Mar 20;15(1):2485.