YHS-12 |
رقم الكتالوجGC92072
One-Click Copy Product Info
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YHS-12 هو الدهون الموجبة الأيونية (pKa = 6.506) التي تم استخدامها في توليد الجسيمات النانوية الدهنية (LNPs) لتوصيل siRNA و mRNA في المختبر وفي الجسم الحي.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2959463-68-2
Sample solution is provided at 25 µL, 10mM.
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YHS-12 is an ionizable cationic lipid (pKa = 6.506) that has been used in the generation of lipid nanoparticles (LNPs) for the delivery of siRNA and mRNA in vitro and in vivo.1 LNPs containing YHS-12 and the macrophage targeting peptide CRVLRSGSC and encapsulating mRNA encoding chimeric antigen receptor targeting methicillin-resistant S. aureus (MRSA) and siRNA targeting caspase-11 increase the phagocytosis rate of MRSA in RAW 264.7 macrophages and primary mouse bone marrow-derived macrophages (BMDMs). Intravenous administration of these LNPs decreases blood bacterial burden and increases survival in a model of sepsis using cyclophosphamide-induced immunosuppressed mice.
References:
[1]. Tang, C., Jing, W., Han, K., et al.mRNA-laden lipid-nanoparticle-enabled in situ CAR-macrophage engineering for the eradication of multidrug-resistant bacteria in a sepsis mouse modelACS Nano18(3)2261-2278(2024).
| Cas No. | 2959463-68-2 | SDF | |
| Chemical Name | (2E)-N1,N4-bis[2-(didodecylamino)ethyl]-2-butenediamide | ||
| Canonical SMILES | CCCCCCCCCCCCN(CCNC(/C=C/C(NCCN(CCCCCCCCCCCC)CCCCCCCCCCCC)=O)=O)CCCCCCCCCCCC | ||
| Formula | C56H112N4O2 | M.Wt | 873.5 |
| الذوبان | Chloroform: Sparingly soluble: 1-10 mg/ml,Methanol: Slightly soluble: 0.1-1 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.1448 mL | 5.7241 mL | 11.4482 mL |
| 5 mM | 229 μL | 1.1448 mL | 2.2896 mL |
| 10 mM | 114.5 μL | 572.4 μL | 1.1448 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)