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BGN3

Catalog No.GC70814 Copy One-Click Copy Product Info

BGN3 is a good substrate for the SNAP-tag and H5 enzymes.

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BGN3 Chemical Structure

Cas No.: 1151762-33-2

Size Price Stock Qty
1 mg
$180.00
In stock
5 mg
$425.00
In stock
10 mg
$594.00
In stock

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Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.



Description of BGN3

BGN3 is a good substrate for the SNAP-tag and H5 enzymes. The activities of SNAP-tag and H5 enzymes on BGN3 are reasonable (IC50= 15.6 and 23.5 μM, respectively). BGN3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.

Substrate specificity of SNAP-tag® and H5 by competitive inhibition method (IC50) by using SVG as substrate[1].

References:
[1]. Merlo R, et al. The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate. J Enzyme Inhib Med Chem. 2021;36(1):85-97.

Chemical Properties of BGN3

Cas No. 1151762-33-2 SDF
Formula C13H12N8O M.Wt 296.29
Solubility DMSO : 100 mg/mL (337.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Storage 4°C, stored under nitrogen
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of BGN3

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.3751 mL 16.8754 mL 33.7507 mL
5 mM 675 μL 3.3751 mL 6.7501 mL
10 mM 337.5 μL 1.6875 mL 3.3751 mL
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**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

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In vivo Formulation Calculator (Clear solution) of BGN3

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % saline
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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Product Documents

Quality Control & SDS

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Reviews

Review for BGN3

Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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