Biotin Azide Plus is an oxazolidine reagent that integrates azide-biotin click chemistry and a photocleavable linker arm. Biotin Azide Plus not only reacts with biotin thioether to form stable sulfinimide products, but also enables bioconjugation of proteins and DNA through biotin redox-activated chemical labeling technology. Taking advantage of the streptavidin capture and photo-release properties, Biotin Azide Plus effectively facilitates the isolation of lipid-derived electrophile-protein adducts, thus finding wide application in scientific research related to fields such as SKBR3 cancer [1] [2].
In Vitro, Bioconjugation Application of Biotin Azide Plus - Protein Labeling[1]: Substrate: Biotinylated Trastuzumab (containing Avi-tag). Conditions: 50 μM protein + 5-fold molar excess of reagent, reaction in PBS at room temperature for 1 h. Bioconjugation Application of Biotin Azide Plus-DNA Labeling[1]: Substrate: 5'-biotinylated single-stranded DNA. Conditions: 5-fold molar excess of reagent, reaction in aqueous solution at room temperature for 1 h. Results: Azide handles are efficiently introduced, while no labeling is observed on non-biotinylated DNA. Biotin Azide Plus exhibits photocleavable properties, and quantitative cleavage is achieved upon irradiation with low-intensity 365 nm ultraviolet light (4 W handheld UV lamp) at room temperature for 1 h[2].
References:
[1]. Cotton AD, et al. Biotin as a Reactive Handle to Selectively Label Proteins and DNA with Small Molecules. ACS Chem Biol. 2022;17(12):3270-3275.
[2]. Kim H Y H, et al. An azido-biotin reagent for use in the isolation of protein adducts of lipid-derived electrophiles by streptavidin catch and photorelease[J]. Molecular & Cellular Proteomics, 2009, 8(9): 2080-2089.
In Vitro, Bioconjugation Application of Biotin Azide Plus - Protein Labeling[1]: Substrate: Biotinylated Trastuzumab (containing Avi-tag). Conditions: 50 μM protein + 5-fold molar excess of reagent, reaction in PBS at room temperature for 1 h. Bioconjugation Application of Biotin Azide Plus-DNA Labeling[1]: Substrate: 5'-biotinylated single-stranded DNA. Conditions: 5-fold molar excess of reagent, reaction in aqueous solution at room temperature for 1 h. Results: Azide handles are efficiently introduced, while no labeling is observed on non-biotinylated DNA. Biotin Azide Plus exhibits photocleavable properties, and quantitative cleavage is achieved upon irradiation with low-intensity 365 nm ultraviolet light (4 W handheld UV lamp) at room temperature for 1 h[2].
References:
[1]. Cotton AD, et al. Biotin as a Reactive Handle to Selectively Label Proteins and DNA with Small Molecules. ACS Chem Biol. 2022;17(12):3270-3275.
[2]. Kim H Y H, et al. An azido-biotin reagent for use in the isolation of protein adducts of lipid-derived electrophiles by streptavidin catch and photorelease[J]. Molecular & Cellular Proteomics, 2009, 8(9): 2080-2089.