DBCO-cyclo (RGDfK) is a clickable DBCO-modified cyclic RGD peptide, and also a DBCO derivative of cyclo (RGDfK). DBCO-cyclo (RGDfK) is synthesized via an amide coupling reaction between DBCO-NHS ester and cyclo (RGDfK). DBCO-cyclo (RGDfK) can react with azide groups through copper-free click chemistry (SPAAC) to immobilize RGD peptides onto biomaterials or hydrogels. The RGD sequence in DBCO-cyclo (RGDfK) is recognizable by cellular integrins and promotes cell adhesion. DBCO-cyclo (RGDfK) can be used in studies related to tissue engineering, 3D cell culture, and surface functionalization of biomaterials [1].
In Vitro, DBCO-cyclo (RGDfK) can react with azide groups in FEFEFKFK peptide hydrogels via SPAAC click chemistry to achieve covalent immobilization of RGD functional groups, without compromising the structural stiffness of the hydrogel[1].
References:
[1]. Curd J. Customisable peptide hydrogels as user-defined biomimetic models of specific tissue microenvironments[M]. University of Nottingham (United Kingdom), 2022.
In Vitro, DBCO-cyclo (RGDfK) can react with azide groups in FEFEFKFK peptide hydrogels via SPAAC click chemistry to achieve covalent immobilization of RGD functional groups, without compromising the structural stiffness of the hydrogel[1].
References:
[1]. Curd J. Customisable peptide hydrogels as user-defined biomimetic models of specific tissue microenvironments[M]. University of Nottingham (United Kingdom), 2022.