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AMC(7-Amino-4-methylcoumarin) (Synonyms: AMC, Coumarin 120, 4-methyl-7-aminocoumarin, NSC 45796)

Katalog-Nr.GA11005

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AMC(7-Amino-4-methylcoumarin) Chemische Struktur

Cas No.: 26093-31-2

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100g
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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 1 publications

Product Documents

Quality Control & SDS

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Background

7-amino-4-methylcoumarin (Coumarin 120) has been used as a fluorogenic probe for many serine proteases.

References:
[1]. Choi MG, et al. A fluorogenic method for measuring enteropeptidase activity: spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin.BMB Rep. 2011 Jul;44(7):458-61.

Chemical Properties

Cas No. 26093-31-2 SDF
Überlieferungen AMC, Coumarin 120, 4-methyl-7-aminocoumarin, NSC 45796
Chemical Name 7-amino-4-methylchromen-2-one
Canonical SMILES CC1=CC(=O)OC2=C1C=CC(=C2)N
Formula C10H9NO2 M.Wt 175.2
Löslichkeit ≥ 17.5mg/mL in DMSO Storage
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 5.7078 mL 28.5388 mL 57.0776 mL
5 mM 1.1416 mL 5.7078 mL 11.4155 mL
10 mM 0.5708 mL 2.8539 mL 5.7078 mL
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Berechnen

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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