BODIPY 505/515-8-C3-COOH is a green fluorescing derivative, as a fluorescent dye for imaging lipid droplets in nannochloropsis. BODIPY 505/515-8-C3-COOH can be used for the research of flow cytometric high-throughput screening and cell sorting.
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| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 5mg | $20.00 | In Stock | 1 | |
| 10mg | $31.00 | In Stock | 1 | |
| 25mg | $61.00 | In Stock | 1 |
BODIPY 505/515-8-C3-COOH is a green fluorescing derivative, as a fluorescent dye for imaging lipid droplets in nannochloropsis. BODIPY 505/515-8-C3-COOH can be used for the research of flow cytometric high-throughput screening and cell sorting[1].
References:
[1]. ChristianSüdfeld, et al. Optimization of high-throughput lipid screening of the microalga Nannochloropsis oceanica using BODIPY 505/515. Algal Research,
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells
1. Dissolve BODIPY 505/515-8-C3-COOH in DMSO at a concentration of 4 mg/mL, then dilute to different working stock concentrations with DMSO.
2. Add an appropriate amount of BODIPY 505/515-8-C3-COOH working stock solution to the cell suspension to achieve a final DMSO volume fraction of 2-10% (v/v) and a final BODIPY 505/515-8-C3-COOH concentration of 0.8-4 μg/mL.
3. After mixing the cells and the dye thoroughly, incubate in the dark for 15 min, then detect using a flow cytometer.