BDP R6G maleimide |
Katalog-Nr.GC59059
One-Click Copy Product Info
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BDP R6G Maleimid ist ein Borodipyrromethan-Fluorophor mit Ähnlichen Absorptions- und EmissionswellenlÄngen wie R6G-Rhodamin.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2183473-32-5
Sample solution is provided at 25 µL, 10mM.
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BDP R6G maleimide is a high-performance, thiol-reactive fluorescent dye with an excitation maximum of 530nm and an emission maximum of 548nm, offering exceptional brightness and photostability. BDP R6G maleimide enables site-specific labeling of cysteine residues in proteins or other thiol-containing biomolecules via its maleimide functional group, which is a critical requirement for precise single-molecule Förster resonance energy transfer (smFRET) and other single-molecule detection techniques. BDP R6G maleimide is suitable for applications such as smFRET, single-molecule tracking (SMT), and fluorescence microscopy of proteins[1].
References:
[1] BenchChem. BDP R6G Maleimide for Single-Molecule Imaging: Application Notes and Protocols. 2025.
Experimental Protocol for Protein Labeling with BDP R6G Maleimide
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
(1) Protein Solution Preparation: Dissolve the target protein in a degassed buffer free of thiol-containing compounds (concentration: 1–10mg/mL, pH=7.0–7.5). If the protein contains disulfide bonds, add a 10- to 100-fold molar excess of TCEP reducing agent and incubate at room temperature for 20–30 minutes.
(2) Dye Stock Solution Preparation: Prepare a 10mM stock solution of BDP R6G maleimide in anhydrous DMSO or DMF. Vortex to dissolve completely and protect from light (recommended to use immediately after preparation).
(3) Labeling Reaction: Add the dye stock solution to the protein solution at a dye-to-protein molar ratio of 10:1 to 20:1. Flush the reaction vial with inert gas, seal tightly, and incubate in the dark with gentle agitation for 2 hours at room temperature or overnight at 4°C.
(4) Purification and Degree of Labeling (DOL) Determination: Remove unreacted dye via gel filtration or HPLC. Measure the absorbance of the conjugate at 530nm (for BDP R6G maleimide) and 280nm (for the protein). Calculate the DOL using the correction formula: A₂₈₀(corrected) = A₂₈₀(measured)–(A₅₃₀×0.18).
Precautions:
(1) Perform all operations protected from light. Aliquot and store the dye stock solution at –20°C.
(2) The protein labeling buffer must be thoroughly degassed to prevent oxidation of thiol groups, which may reduce labeling efficiency.
(3) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] BenchChem. BDP R6G Maleimide for Single-Molecule Imaging: Application Notes and Protocols. 2025.
| Cas No. | 2183473-32-5 | SDF | |
| Formula | C24H21N4BF2O3 | M.Wt | 462.26 |
| Löslichkeit | good in DMF, DMSO, DCM | Storage | -20°C,protect from light. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1633 mL | 10.8164 mL | 21.6328 mL |
| 5 mM | 432.7 μL | 2.1633 mL | 4.3266 mL |
| 10 mM | 216.3 μL | 1.0816 mL | 2.1633 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)