TRPM4-IN-2 (Synonyms: NBA) |
Katalog-Nr.GC68002
One-Click Copy Product Info
|
TRPM4-IN-2 is a potent transient receptor potential melastatin 4 (TRPM4) inhibitor with an IC50 value of 0.16μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 667411-04-3
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
TRPM4-IN-2 is a potent transient receptor potential melastatin 4 (TRPM4) inhibitor with an IC50 value of 0.16μM [1]. TRPM4-IN-2 binds in a pocket formed between the S3, S4, and TRP helices and the S4-S5 linker of TRPM4 to block the TRPM4 channel [2]. TRPM4-IN-2 has been widely used to decrease mouse TRPM4 currents and suppress arrhythmias [3].
In vitro, TRPM4-IN-2 treatment (100μM) for 48 hours significantly inhibited the proliferation of MDA-MB-231, A549 and KYSE30 cells [4]. Pre-treatment of HT22 cells with 10μM TRPM4-IN-2 for 2 hours significantly protected the cells from 5mM glutamate-induced cell death and disruption of cell integrity[5].
References:
[1] Stokłosa P, Borgström A, Hauert B, et al. Investigation of novel small molecular TRPM4 inhibitors in colorectal cancer cells[J]. Cancers, 2021, 13(21): 5400.
[2] Ekundayo B, Arullampalam P, Gerber C E, et al. Identification of a binding site for small molecule inhibitors targeting human TRPM4[J]. Nature communications, 2025, 16(1): 833.
[3] Hu Y, Cang J, Hiraishi K, et al. The role of TRPM4 in cardiac electrophysiology and arrhythmogenesis[J]. International Journal of Molecular Sciences, 2023, 24(14): 11798.
[4] Chang W, Gao W, Luo B, et al. Comprehensive insights into the role of TRPM4 in Pan-cancer progression and immune regulation[J]. ImmunoTargets and Therapy, 2025: 831-848.
[5] Bianchi B, Smith P A, Abriel H. The ion channel TRPM4 in murine experimental autoimmune encephalomyelitis and in a model of glutamate-induced neuronal degeneration[J]. Molecular brain, 2018, 11(1): 41.
| Cell experiment [1]: | |
Cell lines | MDA-MB-231 cells |
Preparation Method | MDA-MB-231 cells were cultivated in 96-well plate at a density of 5×103 cells/well in DMEM medium supplemented with 10% fetal bovine serum (FBS), and 1% penicillin/streptomycin. Cells were treated with different concentrations of the TRPM4-IN-2 (0, 0.1, 1, 10, 50, and 100µM) for 48h. The viability was determined. |
Reaction Conditions | 0, 0.1, 1, 10, 50, and 100µM; 48h |
Applications | TRPM4-IN-2 treatment reduced the viability of MDA-MB-231 cells in a dose-dependent manner. |
References: | |
| Cas No. | 667411-04-3 | SDF | |
| Überlieferungen | NBA | ||
| Formula | C19H14ClNO4 | M.Wt | 355.77 |
| Löslichkeit | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.8108 mL | 14.054 mL | 28.108 mL |
| 5 mM | 562.2 μL | 2.8108 mL | 5.6216 mL |
| 10 mM | 281.1 μL | 1.4054 mL | 2.8108 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)