740 Y-P (trifluoroacetate salt)

740 Y-P is a synthetic phosphopeptide activator of PI3K.1,2 It contains amino acids from the Antennapedia protein, which acts as a cellular internalization sequence, followed by a sequence from PDGFR that is recognized by the SH2 domain of PI3K.1 740 Y-P (15 µM) increases phosphorylation of PI3K, mammalian target of rapamycin (mTOR), and glycogen synthase kinase 3β (GSK3β) in HepG2 hepatocellular carcinoma cells.2 It prevents glucose-oxygen deprivation-induced decreases in viability of primary rat hippocampal cells in an in vitro model of ischemia when used at a concentration of 1 µM.3 740 Y-P (1 µM), in combination with the thrombopoietin receptor (TPOR) agonist butyzamide, hematopoietic stem cell (HSC) self-renewal agonist UM171, and polymer PCL-PVAc-PEG, has been used to expand isolated human umbilical cord blood HSCs ex vivo for up to 30 days.4