EDANS |
| Catalog No.GC61761 |
EDANS (1,5-EDANS) est un substrat fluorogène nouveau et désactivé pour le dosage de la protéase rétrovirale par transfert d'énergie de résonance (RET) .
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 50402-56-7
Sample solution is provided at 25 µL, 10mM.
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EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET)[1].
The peptide sequence of EDANS is derived from a natural processing site for HIV-1 PR[1].Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that was linearly related to the extent of substrate hydrolysis[1].
[1]. E D Matayoshi, et al. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer. Science. 1990 Feb 23;247(4945):954-8.
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