Accueil>>Peptides>>LL-37 (trifluoroacetate salt)

LL-37 (trifluoroacetate salt) (Synonyms: CAP-18, hCAP-18, Cathelicidin, FALL-39)

Catalog No.GC14377

LL-37 (sel de trifluoroacétate) est un peptide antimicrobien dérivé de cathélicidine, amphipathique et composé de 37 résidus, qui présente un large spectre d'activité antimicrobienne.

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LL-37 (trifluoroacetate salt) Chemical Structure

Cas No.: 154947-66-7

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1mg
88,00 $US
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5mg
182,00 $US
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

HaCaT cells

Preparation Method

HaCaT cells were transfected with a 2 μg of empty vector and 0.1, 0.5, 1, or 2 μg of LL-37 plasmids for 6 h and then internalized with P. gingivalis (MOI 100, 6 h) using the antibiotic protection assay. Total RNA was extracted after 18 h.

Reaction Conditions

0.1, 0.5, 1, or 2 μg; 6 h

Applications

The qRT-PCR results showed that compared with the control cells, the number of live P. gingivalis was significantly reduced by 0.79, 0.62, and 0.69 times in cells transfected with 0.5, 1, or 2 μg of LL 37 plasmids, respectively. LL 37 mRNA expression gradually increased significantly as transfected with 0.1, 0.5, or 1 μg of LL 37 plasmids, which was followed by a decrease and reached a peak in cells treated with 1 μg of LL 37 plasmids.

Animal experiment [2]:

Animal models

BALB/c female mice

Preparation Method

Mice were intravenously injected with PBS (200 μl) or LL 37 (3 μg/200 μl in PBS) just prior to CLP or sham operation. Peritoneal exudates (3 ml) and blood were collected 14–16 h after the operation, and the number of bacteria and inflammatory cells was determined.

Dosage form

3 μg/200 μl in PBS; i.v.

Applications

LL 37 administration significantly reduced the bacterial load in the peritoneal exudates (PBS-CLP vs LL-37-CLP) as well as blood.

References:

[1]. Yang X, et al. LL-37-Induced Autophagy Contributed to the Elimination of Live Porphyromonas gingivalis Internalized in Keratinocytes. Front Cell Infect Microbiol. 2020 Oct 15;10:561761.

[2]. Kumagai Y, et al. Antimicrobial peptide LL-37 ameliorates a murine sepsis model via the induction of microvesicle release from neutrophils. Innate Immun. 2020 Oct;26(7):565-579.

Background

LL-37 contient 37 résidus d'acides aminés avec les deux premiers résidus de leucine (L1LGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES37) et existe principalement dans les cellules épithéliales et les neutrophiles.

Une expérience in vitro a montré que le traitement avec 4 et 10 μM de LL-37 réduisait à la fois le nombre et la viabilité des cellules ostéoblastiques humaines MG63. Dans les mêmes conditions in vitro, le prétraitement des pMSCs avec 1 et 10 μg/mL de LL-37 n'a eu aucun effet sur leur capacité migratoire. Cependant, un prétraitement avec 1 μg/mL de LL-37 a augmenté leur potentiel migratoire après 48 heures. Une étude in vitro a indiqué qu'à une concentration de 1 µmol/L de LL-37 pendant 24 heures, celui-ci empêchait l'expression stimulée par LPS du MCP-1 analysée tant au niveau transcriptomique que protéique, mais n'avait aucun effet sur l'expression transcriptomique des récepteurs TLR2 et TLR4. En même temps, un traitement avec des concentrations de LL-37 à hauteur de 0,1 et 1 µmol/L pendant une durée d'une heure dans les cellules PDL induisait une immunoréactivité pour le peptide antimicrobien LL-37.

Une étude in vivo suggère que le traitement par 2 μg/souris de LL-37 par voie intraveineuse améliore la survie des souris septicémiques CLP avec un effet dose-dépendant. LL-37 améliore le niveau d'ectosomes ayant un potentiel antibactérien plus élevé, ce qui réduit la charge bactérienne chez les souris CLP.[1] La libération d'ectosomes à partir de neutrophiles est induite de manière dose-dépendante (1, 3 ou 10 μg/ml) par LL-37. L'injection de LL-37 supprime l'infiltration des cellules polymorphonucléaires chez les souris CLP, où la charge bactérienne et la réponse inflammatoire sont diminuées.[3]

References:
[1].Nagaoka I, et al. Therapeutic Potential of Cathelicidin Peptide LL-37, an Antimicrobial Agent, in a Murine Sepsis Model. Int J Mol Sci. 2020 Aug 19;21(17):5973.
[2].Bankell E, et al. LL-37-induced caspase-independent apoptosis is associated with plasma membrane permeabilization in human osteoblast-like cells. Peptides. 2021 Jan;135:170432.
[3].Kumagai Y, et al. Antimicrobial peptide LL-37 ameliorates a murine sepsis model via the induction of microvesicle release from neutrophils. Innate Immun. 2020 Oct;26(7):565-579.
[4].Oliveira-Bravo M, et al. LL-37 boosts immunosuppressive function of placenta-derived mesenchymal stromal cells. Stem Cell Res Ther. 2016 Dec 30;7(1):189.
[5].Aidoukovitch A, et al. The host defense peptide LL-37 is internalized by human periodontal ligament cells and prevents LPS-induced MCP-1 production. J Periodontal Res. 2019 Dec;54(6):662-670.

Chemical Properties

Cas No. 154947-66-7 SDF
Synonymes CAP-18, hCAP-18, Cathelicidin, FALL-39
Canonical SMILES CC[C@]([C@@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/C/N=C(O)/[C@](/N=C(O)/[C@](N)([H])CC(C)C)([H])CC(C)C)([H])CC(O)=O)([H])CC1=CC=CC=C1)([H])CC2=CC=CC=C2)([H])CCCNC(N)=N
Formula C205H340N60O53 M.Wt 4493.32
Solubility Water: 1 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 0.2226 mL 1.1128 mL 2.2255 mL
5 mM 0.0445 mL 0.2226 mL 0.4451 mL
10 mM 0.0223 mL 0.1113 mL 0.2226 mL
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