GLPBio

Naringinase

Catalog No.: GC61697Purity:>98.00%
COASDSData Sheet

La naringinase, un complexe enzymatique hydrolytique, possède l'activité à la fois de l'α-L-rhamnosidase et de la β-D-glucosidase.

Les produits sont exclusivement destinés à la recherche. Ils ne doivent pas être utilisés chez l’être humain. Nous ne vendons pas aux patients.

Naringinase
Cas No.: 9068-31-9
50mgIn Stock
$15.00
1
100mgIn Stock
$19.00
1
500 mgIn Stock
$42.00
1
1gIn Stock
$59.00
1
5gIn Stock
$146.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

Citations

Aucune citation n’est actuellement disponible pour ce produit. Vous trouverez ci-dessous une sélection de publications citant des produits GlpBio.
  • Nature cover
    Nature
    641, 529–536 (2025)
  • Nature cover
    Nature
    628, 630–638 (2024)
  • Nature cover
    Nature
    632, 686–694 (2024)
  • Nature cover
    Nature
    618, 1017–1023 (2023)
  • Nature cover
    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
  • Science cover
    Science
    388(6745) (2025)
  • Science cover
    Science
    387(6739) (2025)
  • Science cover
    Science
    387(6734) (2025)
  • Cell Research cover
    Cell Research
    35, 97–116 (2025)
  • Cell Research cover
    Cell Research
    34, 683–706 (2024)
  • Cell Research cover
    Cell Research
    33, 273–287 (2023)
  • Cell Research cover
    Cell Research
    33, 546–561 (2023)
  • Cell Research cover
    Cell Research
    33, 904–922 (2023)
  • Cell Research cover
    Cell Research
    31, 1291–1307 (2021)

Description

Naringinase is a complex enzyme with dual activities of α-L-rhamnosidase and β-D-glucosidase. Naringinase can hydrolyze naringin. Naringinase enhances the bioavailability of flavonoids through flavonoid deglycosylation. Naringinase can be used for research on debittering citrus juice, flavonoid deglycosylation, and improving wine flavor[1-4].

In vitro, MFEN (8:1) prepared by co-incubating mulberry fruit extract with Naringinase was used to pretreat IgE-sensitized RBL-2H3 cells at 0-200µg/mL for 1 hour, followed by antigen stimulation for 4 hours. MFEN inhibited β-hexosaminidase and TNF-α release. MFEN reduced PGD2 and LTC4 production. MFEN suppressed phosphorylation of Syk, PLCγ1/2, PKCδ, LAT, ERK1/2, JNK, Gab2, PI3K, Akt, cPLA2, and 5-LO. MFEN suppressed COX-2 expression[5].

In vivo, Kunming mice received oral gavage of 1.00-10.0g/kg Naringinase once daily for 14 days. Naringinase did not cause scratching, jumping, respiratory distress, cyanosis, or paralysis in the mice. Naringinase did not alter fur color, eye color, agility, sensitivity, or defecation in the mice[6].

References:

[1] Bodakowska-Boczniewicz J, Garncarek Z. Use of Naringinase to Modify the Sensory Quality of Foods and Increase the Bioavailability of Flavonoids: A Systematic Review. Molecules. 2025 May 29;30(11):2376.

[2] Hallikeri NS, Yaraguppi DA, Gangadharappa BS, et al. Naringinase: A comprehensive review on its characteristics, production, and biotechnological applications. Enzyme Microb Technol. 2026 Apr;195:110808.

[3] Puri M. Updates on naringinase: structural and biotechnological aspects. Appl Microbiol Biotechnol. 2012 Jan;93(1):49-60.

[4] Salunkhe JD, Patil SV. Improved naringinase double screen plate assay: progress towards the perfect screening. Nat Prod Res. 2025 Feb;39(3):609-612.

[5] Yoo JM, Kim NY, Seo JM, et al. Inhibitory effects of mulberry fruit extract in combination with naringinase on the allergic response in IgE-activated RBL-2H3 cells. International Journal of Molecular Medicine. 2014;33(2):469-477.

[6] Ni H, Chen F, Cai H, et al. Characterization and preparation of Aspergillus niger naringinase for debittering citrus juice. Journal of Food Science. 2012;77(1):C2-C7.

Protocole (Protocol)

Cell experiment [1]:

Cell lines

 RBL-2H3 cells (rat basophilic leukemia cell line)

Preparation Method

 RBL-2H3 cells were maintained in MEM supplemented with 5% FBS, 100U/ml penicillin and 100μg/ml streptomycin at 37°C, 5% CO2. Cells were sensitized with 1μg/ml DNP-IgE for 24h, then treated with Naringinase in combination with mulberry fruit extract (1:8; 0-200μg/mL) for 1h, challenged with 25ng/ml DNP-HSA for 4h; assays included WST-1 cytotoxicity, β-hexosaminidase release, TNF-α ELISA, PGD2/LTB4/LTC4 EIA, and immunoblot for phosphorylation of Syk/Lyn/Fyn/PLCγ1/2/PKCδ/LAT/ERK1/2/JNK/Gab2/PI3K/Akt/cPLA2/5-LO and COX-2 expression.

Reaction Conditions

 0-200μg/mL (Naringinase : mulberry fruit extract=1:8); 1h pretreatment + 4h antigen challenge

Applications

 Naringinase in combination with mulberry fruit extract (MFEN) inhibited β-hexosaminidase and TNF-α release, reduced PGD2 and LTC4 formation, suppressed phosphorylation of Syk/PLCγ1/2/PKCδ/LAT/ERK1/2/JNK/Gab2/PI3K/Akt/cPLA2/5-LO, and downregulated COX-2 expression in IgE-activated RBL-2H3 cells, with no cytotoxicity in the tested range.
Animal experiment [2]:

Animal models

 Kunming strain mice

Preparation Method

Mice were acclimated for 7d at 21 to 25°C, 40% to 70% humidity, fasted 5h, then orally gavaged once daily for 14 days with Naringinase powder dissolved in purified water at 1.00, 2.15, 4.64, or 10.0g/kg; general health, toxicity symptoms, mortality, body weight, and food intake were monitored 3 times daily during administration.

Dosage form

1.00-10.0g/kg; p.o.; once daily for 14 days

Applications

Naringinase powder administration caused no mortality, no toxicity symptoms (scratching, jumping, respiratory distress, cyanosis, paralysis), no abnormal fur/eye color or action, and no significant difference in body weight gain or food intake compared with control in Kunming mice.

References:

[1] Yoo JM, Kim NY, Seo JM, et al. Inhibitory effects of mulberry fruit extract in combination with naringinase on the allergic response in IgE-activated RBL-2H3 cells. International Journal of Molecular Medicine. 2014;33(2):469-477.

[2] Ni H, Chen F, Cai H, et al. Characterization and preparation of Aspergillus niger naringinase for debittering citrus juice. Journal of Food Science. 2012;77(1):C2-C7.

Documents produit (Product Documents)

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Propriétés chimiques (Chemical Properties)

N° CAS
9068-31-9
SMILES
[Naringinase]
Solubilité
Water: 120 mg/mL
Conservation
Store at -20°C
Conseils généraux
Choisissez un solvant adapté selon les données de solubilité du produit. Après préparation, répartissez la solution mère en aliquotes afin d’éviter les cycles répétés de congélation-décongélation.
Utilisez les solutions mères dans les 6 mois si elles sont conservées à -80°C, ou dans le mois si elles sont conservées à -20°C.
Pour améliorer la solubilité, réchauffez le tube à 37°C et soniquez-le pendant une durée appropriée.
Conditions d’expédition
Les échantillons d’évaluation sont expédiés avec des blocs réfrigérants. Les autres formats sont expédiés à température ambiante ou, sur demande, avec des blocs réfrigérants.

Calculateurs (Calculators)Molarité / Dilution / Masse moléculaire / Conversion d’unités

g/mol

Reviews

Review for Naringinase

Rating: 5
  • 5 Star100%
  • 4 Star0%
  • 3 Star0%
  • 2 Star0%
  • 1 Star0%