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Pyrene azide 1

Catalog No.GC13260 Copy One-Click Copy Product Info

Fluorescent probes

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Pyrene azide 1 Chemical Structure

Taille Prix Stock Qté
5mg
329,00 $US
En stock
25mg
1 028,00 $US
En stock

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Sample solution is provided at 25 µL, 10mM.



Description of Pyrene azide 1

Pyrene is a polyaromatic hydrocarbon with strong short-wavelength fluorescence. Unlike other fluorescent dyes, polyaromatic hydrocarbons are fluorescent probes with strong sensitivity to microenvironment. Thus, its fluorescence is different in polar, and nonpolar environments. Other effects can also be observed. When two pyrenes are in close proximity, they form excimers. Excimer formation can be easily observed, and quantitatively estimated using fluorescent spectra. Pyrene azide is a reagent for easy pyrene Click Chemistry labeling of any alkyne-bearing molecule. It allows to turn any molecule into pyrene-bearing probe. This azide contains hydrophilic triethyleneglycol linker to mitigate intrinsic pyrene hydrophobicity, and facilitate attachment to biomolecules in aqueous solutions.

Chemical Properties of Pyrene azide 1

Cas No. SDF
Chemical Name N-[2-[2-(2-azidoethoxy)ethoxy]ethyl]pyrene-1-carboxamide
Canonical SMILES C1=CC2=C3C(=C1)C=CC4=C(C=CC(=C43)C=C2)C(=O)NCCOCCOCCN=[N+]=[N-]
Formula C23H22N4O3 M.Wt 402.45
Solubility soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile Storage 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Pyrene azide 1

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1 mg 5 mg 10 mg
1 mM 2.4848 mL 12.4239 mL 24.8478 mL
5 mM 497 μL 2.4848 mL 4.9696 mL
10 mM 248.5 μL 1.2424 mL 2.4848 mL
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In vivo Formulation Calculator (Clear solution) of Pyrene azide 1

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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