TRAP-5 amide (Synonyms: SFLLR-NH2) |
Catalog No.GA23637
One-Click Copy Product Info
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TRAP-5 amide, also known as SFLLR-amide, is a protease-activated receptor 1 (PAR 1) agonist peptide.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 141923-41-3
Sample solution is provided at 25 µL, 10mM.
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TRAP-5 amide, also known as SFLLR-amide, is a protease-activated receptor 1 (PAR 1) agonist peptide[1]. PAR 1 is the primary thrombin-responsive receptor in human cells and plays a significant role in tissue injury responses and related inflammation processes[2]. TRAP-5 amide mimics the action of thrombin and activates the thrombin receptor PAR 1 without the need for proteolysis, making it useful for studying PAR 1 activation mechanisms[3]. TRAP-5 amide is the smallest peptide that can release serotonin from human platelets and retain full activity, with the sequence Ser-Phe-Leu-Leu-Arg-NH2[4].
In vitro, treatment of A549 cells with TRAP-5 amide (10 μM) for 30 minutes under static conditions resulted in a positive dynamic mass redistribution (DMR) similar to that caused by a saturating dose of thrombin (10 units/ml), and this effect lasted for at least 2 hours. Additionally, TRAP-5 amide and thrombin showed mutual cross-desensitization[3].
References:
[1] Ceruso M A, McComsey D F, Leo G C, et al. Thrombin receptor-activating peptides (TRAPs): Investigation of bioactive conformations via structure–activity, spectroscopic, and computational studies[J]. Bioorganic & medicinal chemistry, 1999, 7(11): 2353-2371.
[2] Chen* C, Maryanoff* B E, Andrade-Gordon P. Thrombin receptor modulators: Medicinal chemistry, biological evaluation, and clinical application[J]. Thrombin: Physiology and Disease, 2009: 205-236.
[3] Goral V, Wu Q, Sun H, et al. Label-free optical biosensor with microfluidics for sensing ligand-directed functional selectivity on trafficking of thrombin receptor[J]. FEBS letters, 2011, 585(7): 1054-1060.
[4] Sabo T, Gurwitz D, Motola L, et al. Structure-activity studies of the thrombin receptor activating peptide[J]. Biochemical and biophysical research communications, 1992, 188(2): 604-610.
Cell experiment [1]: | |
Cell lines | A549 cells |
Preparation method | The dynamic mass redistribution (DMR) of A549 cells responding to TRAP-5 amide (10μM) obtained under the static and flow conditions, continuous for 30 min. |
Reaction Conditions | 10μM; 30 min |
Applications | Under the static stimulation condition both TRAP-5 amide (10μM) and thrombin (10unit/ml), each at a saturating dose, resulted in a similar positive-DMR that is sustained for at least 2 h. |
References: | |
| Cas No. | 141923-41-3 | SDF | |
| Synonyms | SFLLR-NH2 | ||
| Chemical Name | H-SER-PHE-LEU-LEU-ARG-NH2 | ||
| Formula | C30H51N9O6 | M.Wt | 633.79 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.5778 mL | 7.889 mL | 15.7781 mL |
| 5 mM | 315.6 μL | 1.5778 mL | 3.1556 mL |
| 10 mM | 157.8 μL | 788.9 μL | 1.5778 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)