AF568 is a synthetic fluorophore structurally and functionally identical to Alexa Fluor™ 568, a member of the well-known Alexa Fluor™ family of fluorescent dyes. The excitation peak of AF568 lies at 572 nm and its emission peak is at 598 nm. It can be excited using a 561 nm laser. AF568 is spectrally similar to Texas Red, TF4 (Tide Fluor™ 4), and sulfo-Cyanine3.5 dyes.
As AF568 NHS Ester can be conjugated with proteins and peptides, it is recommended for stable signal generation in imaging, including Western Blotting, fluorescence microscopy, and flow cytometry.