Hydrogel synthesis[1]
This protocol only provides a guideline, and should be modified according to your specific needs.
1、Hydrogels cross-linked with (3-aminopropyl)dimethylmethoxysilane were prepared by blending natural polymers (k-carrageenan (k-CG) and sodium alginate (SA)) with a synthetic polymer (polyvinyl alcohol (PVA)) (MW: 65000g/mol) using the solution casting technique.
2、Initially, k-CG (0.25g) and SA (0.25g) were each dissolved in 30mL of distilled water. The two solutions were then mixed and stirred for 1h.
3、Subsequently, PVA (0.5g) was dissolved in 30mL of distilled water and transferred to the aforementioned polymeric solution, where it was blended for 1.5h.
4、In the next step, Polyamidoamine Dendrimers (PAA) (60µL) was diluted in 3mL of methanol and added to the mixture, followed by stirring for over 1h. Thereafter, different amounts of (3-aminopropyl)dimethylmethoxysilane (80, 160, 240 and 320µL) were each diluted in 5mL of ethanol and added to the mixture.
5、After stirring at 70°C for 3h, the mixture was poured into Petri dishes and dried at room temperature.
6、The hydrogels were named CAP (control), CAP-8, CAP-16, CAP-24, and CAP-32. CAP (control) contained no (3-aminopropyl)dimethylmethoxysilane, while CAP-8, CAP-16, CAP-24, and CAP-32 contained 80, 160, 240 and 320µL of (3-aminopropyl)dimethylmethoxysilane cross-linker, respectively. The entire hydrogel fabrication process was completed at 70°C with continuous stirring.
References:
[1] Anees Ur Rehman Qureshi M, Arshad N, Rasool A, Janjua NK, Butt MS, Naqeeb Ur Rehman Qureshi M, Ismail H. Kappa-carrageenan and sodium alginate-based pH-responsive hydrogels for controlled release of methotrexate. R Soc Open Sci. 2024 Apr 24;11(4):231952. |
