GLPBio

Nuclease P1

Catalog No.: GF05226
COASDSData Sheet

Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid.

Products are for research use only. Not for human use. We do not sell to patients.

Nuclease P1
Cas No.: 54576-84-0
250UIn Stock
$401.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

Citations

No citations are currently available for this product. Below are selected publications citing GlpBio products.
  • Nature cover
    Nature
    641, 529–536 (2025)
  • Nature cover
    Nature
    628, 630–638 (2024)
  • Nature cover
    Nature
    632, 686–694 (2024)
  • Nature cover
    Nature
    618, 1017–1023 (2023)
  • Nature cover
    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
  • Science cover
    Science
    388(6745) (2025)
  • Science cover
    Science
    387(6739) (2025)
  • Science cover
    Science
    387(6734) (2025)
  • Cell Research cover
    Cell Research
    35, 97–116 (2025)
  • Cell Research cover
    Cell Research
    34, 683–706 (2024)
  • Cell Research cover
    Cell Research
    33, 273–287 (2023)
  • Cell Research cover
    Cell Research
    33, 546–561 (2023)
  • Cell Research cover
    Cell Research
    33, 904–922 (2023)
  • Cell Research cover
    Cell Research
    31, 1291–1307 (2021)

Description

Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid. Nuclease P1 is one of the most well-known single stranded specific nucleases in the field of molecular biology, it is widely used in the pharmaceutical and food industries[1]. Nuclease P1 can be obtained by fermentation of Penicillium citrinum: through extraction process, ultrafiltration concentration, drying and purification, etc.

References:
[1]. Mitiushkina NV, et al. Preparation of Duplex Sequencing Libraries for Archival Paraffin-Embedded Tissue Samples Using Single-Strand-Specific Nuclease P1. Int J Mol Sci. 2022 Apr 21;23(9):4586.

Protocol

Reaction Conditions

pH Range: Effective pH Range: 4.0-8.0, Optimal pH Range: 4.5-5.5
Temperature Range: Effective Temperature Range: 35°C-85°C, Optimal Temperature Range: 65°C-75°C

Usage Method

Using yeast nucleic acid (RNA) as substrate: substrate concentration 2%-5%, recommended enzyme dosage 2%-4% (based on substrate dry matter), hydrolysis time 7-9 hours under optimal conditions;
Using yeast as substrate: yeast concentration 10%-12%, nuclease dosage 0.3%-0.5% (based on yeast dry matter), hydrolysis time 7-9 hours under optimal conditions.

Note: Due to differences in application areas and experimental parameters, the actual addition method and dosage of this product should be determined experimentally.

Product Documents

Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
54576-84-0
Solubility
H2O : 4.17 mg/mL (ultrasonic and warming and heat to 37°C)
Storage
Store at 4°C , do not freeze.
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion

g/mol

Reviews

Review for Nuclease P1

Rating: 5
  • 5 Star100%
  • 4 Star0%
  • 3 Star0%
  • 2 Star0%
  • 1 Star0%