N6-Methyladenosine-5-Triphosphate (N6-methyl ATP; m6ATP) is a base-modified analog of adenosine, identified as a minor nucleoside in natural RNA[1]. N6-Methyladenosine-5’-Triphosphate is a dynamic, reversible, covalent modification of ribonucleotides, commonly found in eukaryotic messenger RNA (mRNA), and plays a crucial regulatory role in RNA splicing, export, stability, and translation[2, 3]. N6-Methyladenosine-5-Triphosphate serves as a substrate for RNA polymerase and is used as a complete substitute for ATP in mRNA modification reactions[4, 5]. Additionally, N6-Methyladenosine-5-Triphosphate is an effective agonist for P2Y purinergic receptors in guinea pigs and Escherichia coli[6]. This product is provided in the form of a lithium salt solution.
References:
[1] Yuan Y, Arneson R, Burke E, et al. Efficient 3′-end tailing of RNA with modified adenosine for nanopore direct total RNA sequencing[J]. bioRxiv, 2024: 2024.02. 24.581884.
[2] Zheng H, Li S, Zhang X, et al. Functional implications of active N6-methyladenosine in plants[J]. Frontiers in Cell and Developmental Biology, 2020, 8: 291.
[3] Anderson S J, Kramer M C, Gosai S J, et al. N6-methyladenosine inhibits local ribonucleolytic cleavage to stabilize mRNAs in Arabidopsis[J]. Cell reports, 2018, 25(5): 1146-1157. e3.
[4] Parr C J C, Wada S, Kotake K, et al. N 1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells[J]. Nucleic Acids Research, 2020, 48(6): e35-e35.
[5] Potapov V, Fu X, Dai N, et al. Base modifications affecting RNA polymerase and reverse transcriptase fidelity[J]. Nucleic Acids Research, 2018, 46(11): 5753-5763.
[6] Burnstock G, Fischer B, Hoyle C H V, et al. Structure activity relationships for derivatives of adenosine‐5′‐triphosphate as agonists at P2 purinoceptors: Heterogeneity within P2x and P2y subtypes[J]. Drug development research, 1994, 31(3): 206-219.