306Oi10 |
| カタログ番号GC52090 |
306Oi10 は、メッセンジャー RNA の送達用の脂質ナノ粒子 (LNP) の構築に使用できる分枝鎖のイオン化可能なリピドイドです。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2322290-93-5
Sample solution is provided at 25 µL, 10mM.
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306Oi10 is a branched-chain ionizable lipidoid.1 It has been used in the generation of lipid nanoparticles (LNPs). It has strong surface ionization at pH 5, which is the pH in the late endosomal compartment, and a surface pKa value of 6.4 when encapsulating mRNA. LNPs containing 306Oi10 and encapsulating an mRNA reporter accumulate primarily in the mouse liver and those encapsulating erythropoietin increase serum erythropoietin levels in mice. LNPs containing 306Oi10 and encapsulating mRNA encoding the Cas9 nuclease (mCas9) and single-guide RNA targeting LoxP (sgLoxP) have been used to induce CRISPR-mediated gene editing in Ai9 mice that express a floxed STOP codon to prevent tdTomato expression.2
1.Hajj, K.A., Ball, R.L., Deluty, S.B., et al.Branched-tail lipid nanoparticles potently deliver mRNA in vivo due to enhanced ionization at endosomal pHSmall15(6)e1805097(2019) 2.Hajj, K.A., Melamed, J.R., Chaudhary, N., et al.A potent branched-tail lipid nanoparticle enables multiplexed mRNA delivery and gene editing in vivoNano Lett.20(7)5167-5175(2020)
| Cas No. | 2322290-93-5 | SDF | |
| Canonical SMILES | O=C(CCN(CCCN(CCCN(CCC(OCCCCCCCC(C)C)=O)CCC(OCCCCCCCC(C)C)=O)C)CCC(OCCCCCCCC(C)C)=O)OCCCCCCCC(C)C | ||
| Formula | C59H115N3O8 | M.Wt | 994.6 |
| 溶解度 | DMF: 20 mg/ml,DMSO: 33 mg/ml,Ethanol: 33 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.0054 mL | 5.0271 mL | 10.0543 mL |
| 5 mM | 0.2011 mL | 1.0054 mL | 2.0109 mL |
| 10 mM | 0.1005 mL | 0.5027 mL | 1.0054 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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