Biotin-7-GMP lithium solution (100 mM ± 3 mM)

Biotin-7-GMP (5'-Biotin-G-monophosphate) lithium solution (100 mM ± 3 mM) is a 5'-biotinylated guanosine monophosphate reagent. Biotin-7-GMP lithium solution (100 mM ± 3 mM) covalently links biotin to specific positions (G bases ) of RNA molecules, enabling efficient and specific purification and detection of target RNAs through the ultra-stable binding between biotin and streptavidin ^{[1] [2]}.

In Vitro, Biotin-7-GMP (4-fold molar excess) lithium solution enables the in vitro transcription synthesis of 5'-biotinylated RNA_R9D-14T and RNA_mut aptamers^[1]. Biotin-7-GMP (3 mM; overnight) supports the generation of 5'-end biotinylated RNA aptamers via in vitro transcription at 37°C for use in β2-adrenoceptor binding studies^[2].

References:
[1]. Bompiani KM, et al. A high affinity, antidote-controllable prothrombin and thrombin-binding RNA aptamer inhibits thrombin generation and thrombin activity. Journal of thrombosis and haemostasis: JTH. 2012 May;10(5):870-80.
[2]. Kahsai AW, et al. Conformationally selective RNA aptamers allosterically modulate the β2-adrenoceptor. Nature chemical biology. 2016 Sep;12(9):709-16.