Ferfluor-1 is a Ferroptosis inhibitor ( EC 50 of 57 nM in HT108 cells; EC 50 of 75 nM in OS-RC-2 cells; EC 50 of 2.3 nM in SH-SY5Y neuroblastoma cells), and ratiometric photoluminescent probe with blood-brain barrier permeability. Ferfluor-1 is a specific indicator for the fluctuation of Ferroptosis. Ferfluor-1 alleviates brain disorder diseases of in vivo stroke and PD models [1].
In Vivo, Ferfluor-1 (10 mg/kg; i.v.; single administration) effectively alleviates cerebral ischemic damage in MCAO mice by inhibiting ferroptosis, as evidenced by reduced infarct volume, improved cerebral blood flow, and decreased markers of lipid peroxidation and ferroptosis[1].
In Vitro, Ferfluor-1 potently inhibits ferroptosis in HT1080 fibrosarcoma cells with an EC50 of 57 nM, in OS-RC-2 renal carcinoma cells with an EC50 of 75 nM, and in SH-SY5Y neuroblastoma cells with an EC50 of 2.3 nM[1]. Ferfluor-1 (5 μM) does not inhibit Staurosporine -induced apoptosis in SH-SY5Y neuroblastoma cells[1].
References:
[1]. Yan J, et al. A Druglike Ferrostatin-1 Analogue as a Ferroptosis Inhibitor and Photoluminescent Indicator. Angew Chem Int Ed Engl. 2025;64(20):e202502195.
In Vivo, Ferfluor-1 (10 mg/kg; i.v.; single administration) effectively alleviates cerebral ischemic damage in MCAO mice by inhibiting ferroptosis, as evidenced by reduced infarct volume, improved cerebral blood flow, and decreased markers of lipid peroxidation and ferroptosis[1].
In Vitro, Ferfluor-1 potently inhibits ferroptosis in HT1080 fibrosarcoma cells with an EC50 of 57 nM, in OS-RC-2 renal carcinoma cells with an EC50 of 75 nM, and in SH-SY5Y neuroblastoma cells with an EC50 of 2.3 nM[1]. Ferfluor-1 (5 μM) does not inhibit Staurosporine -induced apoptosis in SH-SY5Y neuroblastoma cells[1].
References:
[1]. Yan J, et al. A Druglike Ferrostatin-1 Analogue as a Ferroptosis Inhibitor and Photoluminescent Indicator. Angew Chem Int Ed Engl. 2025;64(20):e202502195.