IDH1 ligand 1 (Compound 18) can serve as a negative control for the IDH1 target, with no detectable affinity for wild-type IDH1 and an IC50 >10,000 nM [1].
In Vitro, IDH1 ligand 1 (Compound 18) (30 μM) inhibits wild-type IDH1 with an IC50 of >10,000 nM in a fluorescence activity biochemical assay[1].
References:
[1]. Jakob CG, et al. Novel Modes of Inhibition of Wild-Type Isocitrate Dehydrogenase 1 (IDH1): Direct Covalent Modification of His315. J Med Chem. 2018;61(15):6647-6657.
In Vitro, IDH1 ligand 1 (Compound 18) (30 μM) inhibits wild-type IDH1 with an IC50 of >10,000 nM in a fluorescence activity biochemical assay[1].
References:
[1]. Jakob CG, et al. Novel Modes of Inhibition of Wild-Type Isocitrate Dehydrogenase 1 (IDH1): Direct Covalent Modification of His315. J Med Chem. 2018;61(15):6647-6657.