12-Deoxywithastramonolide (Synonyms: Baimantuoluoside C aglycone, 27-Hydroxywithanolide B) |
Catalog No.GC18531
One-Click Copy Product Info
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12-Deoxywithastramonolide는 ashwagandha(W.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 60124-17-6
Sample solution is provided at 25 µL, 10mM.
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12-Deoxywithastramonolide is a natural lactone found in ashwagandha (W.somnifera)[1].
In vitro, 12-Deoxywithastramonolide (0-200µM; 24 or 48h) exhibited low cytotoxicity in J774A.1 macrophages[2]. 12-Deoxywithastramonolide (10µM; 24h) significantly inhibited NO production in LPS-activated J774A.1 macrophages[2]. 12-Deoxywithastramonolide (1.25-10µM; 24h) dose-dependently increased erythrocyte membrane stability and inhibited protein denaturation, with an IC₅₀ of 8.16µM[2].
In vivo, a single oral dose of 12-Deoxywithastramonolide (20mg/kg) significantly attenuated acute thermal nociception in mice, as evidenced by prolonged withdrawal latency to thermal stimulus[2]. A single oral dose of 12-Deoxywithastramonolide (20mg/kg) reduced acetic acid-induced abdominal constrictions in mice (writhing response), exhibiting a 32% inhibition[2]. A single oral dose of 12-Deoxywithastramonolide (20mg/kg) inhibited rat paw edema with a rate of approximately 38%[2].
References:
[1] Nile SH, Nile A, Gansukh E, Baskar V, Kai G. Subcritical water extraction of withanosides and withanolides from ashwagandha (Withania somnifera L) and their biological activities. Food Chem Toxicol. 2019;132:110659.
[2] Chandan G, Kumar C, Chibber P, et al. Evaluation of analgesic and anti-inflammatory activities and molecular docking analysis of steroidal lactones from Datura stramonium L. Phytomedicine. 2021;89:153621.
| Cell experiment [1]: | |
Cell lines | J774A.1 mouse BALB/c macrophages |
Preparation Method | Following 24h incubation, macrophages were incubated for 2h with 100ng/mL LPS, preceded by 12-Deoxywithastramonolide (10µM) treatment at 37°C in 5% CO2. Diclofenac (10µM) was used as positive control. After 2, 12 and 24h of lactone treatment, supernatant was collected and kept at -80°C for the quantification of nitric oxide (NO) using the Griess reaction with slight modifications. |
Reaction Conditions | 10µM; 24h |
Applications | 12-Deoxywithastramonolide significantly inhibited NO production in LPS-activated macrophages. |
| Animal experiment [1]: | |
Animal models | Mice |
Preparation Method | Tail-flick assay: The mouse tail (3-5cm) was kept on the analgesiometer nichrome wire after that the reaction time was measured in terms of tail removal. A reaction time was recorded as the interval between tail exposure to the hot beam and the tail withdrawal reflex. Reaction times to analyze pain were recorded at 0-4h at different intervals after the oral administration of 12-Deoxywithastramonolide (20mg/kg). A cut-off time of 20s was enacted as a protection against skin damage. Diclofenac at a dose of 50 mg/kg was used as a reference drug. |
Dosage form | 20mg/kg; p.o.; once |
Applications | 12-Deoxywithastramonolide significantly decreased the acute thermal nociception in mice (0.5-2h) and extended the animal’s response time to the temperature incitement when compared to the vehicle-treated control. |
References: | |
| Cas No. | 60124-17-6 | SDF | |
| Synonyms | Baimantuoluoside C aglycone, 27-Hydroxywithanolide B | ||
| Chemical Name | 6α,7α-epoxy-5α,22R,27-trihydroxy-1-oxo-ergosta-2,24-dien-26-oic acid δ-lactone | ||
| Canonical SMILES | CC(C1)=C(CO)C(O[C@@]1([H])[C@@H](C)[C@@]2([H])CC[C@@]3([H])[C@]4([H])[C@H]5[C@H](O5)[C@@]([C@@]6(C)[C@@]4([H])CC[C@@]32C)(O)CC=CC6=O)=O | ||
| Formula | C28H38O6 | M.Wt | 470.6 |
| Solubility | DMF: 2 mg/ml,DMSO: 0.1 mg/ml,Ethanol: 20 mg/ml,Ethanol:PBS (pH 7.2)(1:2): 0.3 mg/ml | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1249 mL | 10.6247 mL | 21.2495 mL |
| 5 mM | 425 μL | 2.1249 mL | 4.2499 mL |
| 10 mM | 212.5 μL | 1.0625 mL | 2.1249 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 31 reference(s) in Google Scholar.)