8-Deoxylactucin

8-Deoxylactucin is an orally active sesquiterpene lactone. 8-Deoxylactucin inhibits LPS -induced NO production in RAW264.7 macrophages with an IC₅₀ value of 4.35 μM. 8-Deoxylactucin exerts anti-inflammatory effects by blocking the NF-κB pathway. 8-Deoxylactucin demonstrates hepatoprotective efficacy in LPS/D-galactosamine-induced acute hepatitis model of mice. 8-Deoxylactucin can be used for the study of inflammatory diseases and inflammatory liver injuries ^[1].

In Vivo, 8-Deoxylactucin (2-10 mg/kg, i.g., once daily, 5 days) significantly reduces LPS/D-galactosamine (D-GalN)-induced increase in liver index (liver weight/body weight) in mice^[1].

In Vitro, 8-Deoxylactucin (1 h pretreatment) significantly inhibits LPS -induced NO production in RAW264.7 macrophages, with an IC₅₀ of 4.35 μM^[1]. 8-Deoxylactucin (1-10 μM, 1 h pretreatment) significantly reduces LPS-induced mRNA and protein expression of iNOS, as well as mRNA expression of pro-inflammatory cytokines including IL-1β, IL-6, and TNF-α in RAW264.7 cells^[1]. 8-Deoxylactucin (1-10 μM, 2 h pretreatment) significantly blocks LPS-induced phosphorylation of IKKα/β and IκBα, inhibits IκBα degradation, and reduces nuclear accumulation of NF-κB p65 in RAW264.7 macrophages^[1]. 8-Deoxylactucin (1-10 μM, 1 h pretreatment) significantly enhances protein expression and nuclear translocation of NRF2, up-regulates protein expression of NRF2-targeted antioxidant genes (HO, NQO1), and reduces ROS production in RAW264.7 cells^[1].

References:
[1]. Zhou Y, et al. Sesquiterpene lactones from Cichorium intybus exhibit potent anti-inflammatory and hepatoprotective effects by repression of NF-κB and enhancement of NRF2. J Ethnopharmacol. 2025 Mar 13;343:119439.