Ferrichrome (iron-free) (Synonyms: Desferrichrome) |
Catalog No.GC18325
One-Click Copy Product Info
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Ferrichrome (iron-free) is a ferric ionophore for Ustilago sphaerogena, which can serve as a source of iron for the enzyme ferrochelatase.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 34787-28-5
Sample solution is provided at 25 µL, 10mM.
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Ferrichrome (iron-free) is a ferric ionophore for Ustilago sphaerogena, which can serve as a source of iron for the enzyme ferrochelatase [1]. Ferrichrome can induce the adaptive growth of fission yeast on high ammonium medium and restore the iron-dependent pathways to compensate for the dysfunction of the vacuoles induced by ammonium [2]. Ferrichrome has been widely used to induce the iron transporter Arn1p from the endosomal pathway to the plasma membrane pathway in Saccharomyces cerevisiae[3].
In vitro, Ferrichrome treatment for 24 hours significantly inhibited HT29 cells, SKCO1 cells, and SW480 cells, with IC50 values of 0.02µg/ml, 0.34µg/ml, and 0.38µg/ml, respectively[4]. Treatment with 100µg/ml Ferrichrome for 72 hours significantly induced apoptosis in MKN-45 cells, accompanied by an increase in the expression levels of cleaved PARP and cleaved caspase-9[5]. Treatment with 10µg/ml Ferrichrome for 48 hours significantly increased the expression level of DDIT3 mRNA in SW620 cells, stimulated the activity of the JNK signaling pathway, and promoted cell death[6].
In vivo, Ferrichrome treatment via intraperitoneal injection at a dose of 10mg/kg/day for 8days significantly reduced tumor growth in mice with SUIT-2 xenografts[7].
References:
[1] Straka J G, Emergy T. The role of ferrichrome reductase in iron metabolism of Ustilago sphaerogena[J]. Biochimica et Biophysica Acta (BBA)-Enzymology, 1979, 569(2): 277-286.
[2] Chiu P C, Nakamura Y, Nishimura S, et al. Ferrichrome, a fungal-type siderophore, confers high ammonium tolerance to fission yeast[J]. Scientific reports, 2022, 12(1): 17411.
[3] Kim Y, Yun C W, Philpott C C. Ferrichrome induces endosome to plasma membrane cycling of the ferrichrome transporter, Arn1p, in Saccharomyces cerevisiae[J]. The EMBO Journal, 2002, 21(14): 3632-3642.
[4] Iwama T, Fujiya M, Konishi H, et al. Bacteria‐derived ferrichrome inhibits tumor progression in sporadic colorectal neoplasms and colitis‐associated cancer[J]. Cancer cell international, 2021, 21(1): 21.
[5] Ijiri M, Fujiya M, Konishi H, et al. Ferrichrome identified from Lactobacillus casei ATCC334 induces apoptosis through its iron-binding site in gastric cancer cells[J]. Tumor Biology, 2017, 39(6): 1010428317711311.
[6] Konishi H, Fujiya M, Tanaka H, et al. Probiotic-derived ferrichrome inhibits colon cancer progression via JNK-mediated apoptosis[J]. Nature communications, 2016, 7(1): 12365.
[7] Kita A, Fujiya M, Konishi H, et al. Probiotic-derived ferrichrome inhibits the growth of refractory pancreatic cancer cells[J]. International journal of oncology, 2020, 57(3): 721-732.
| Cell experiment [1]: | |
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Cell lines |
SW480 cells |
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Preparation Method |
SW480 cells were seeded in 96-well plates at a density of 1×104 cells in high-glucose DMEM medium with 10% (v/v) fetal bovine serum (FBS), 2mM L-glutamine, 50U/ml penicillin, and 50µg/ml streptomycin in a humidified atmosphere containing 5% CO2. After 24h of treatment with different concentrations of Ferrichrome (0, 0.1, 1, 2.5, and 5µg/ml), the cell viability was measured. |
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Reaction Conditions |
0, 0.1, 1, 2.5, and 5µg/ml; 24h |
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Applications |
Ferrichrome treatment significantly inhibited the cell viability of SW480 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
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Animal models |
Male BALB/c mice |
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Preparation Method |
Male BALB/c mice (6 weeks old; 22-25g) were housed singly in a standard environment with food and water ad libitum. SUIT-2 cells (1×106/0.1ml) were injected subcutaneously into the back of male BALB/c nude mice. PBS for the studies evaluating the effects of Ferrichrome compared with PBS, Ferrichrome (10mg/kg/day) treatment was intraperitoneally administered after the injection of SUIT-2 cells for 8 days. The tumor volume was analyzed. |
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Dosage form |
10mg/kg/day for 8 days; i.p. |
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Applications |
Ferrichrome treatment significantly reduced tumor growth in mice with SUIT-2 xenografts. |
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References: |
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| Cas No. | 34787-28-5 | SDF | |
| Synonyms | Desferrichrome | ||
| Chemical Name | cyclo(glycylglycylglycyl-N5-acetyl-N5-hydroxy-L-ornithyl-N5-acetyl-N5-hydroxy-L-ornithyl-N5-acetyl-N5-hydroxy-L-ornithyl) | ||
| Canonical SMILES | O=C([C@H](CCCN(C(C)=O)O)NC([C@H](CCCN(C(C)=O)O)NC(CN1)=O)=O)N[C@@H](CCCN(C(C)=O)O)C(NCC(NCC1=O)=O)=O | ||
| Formula | C27H45N9O12 | M.Wt | 687.7 |
| Solubility | Water: 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4541 mL | 7.2706 mL | 14.5412 mL |
| 5 mM | 290.8 μL | 1.4541 mL | 2.9082 mL |
| 10 mM | 145.4 μL | 727.1 μL | 1.4541 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)