1,8-Dihydroxy-4,5-dinitroanthraquinone (ARDP0006; DHDNE) is a potent inhibitor of serine proteinase NS2B/3, the dengue viral protein. 1,8-Dihydroxy-4,5-dinitroanthraquinone has intermolecular proteinase activity and viral inhibition ability with IC50s of 432 渭M and 4.2 渭M, respectively. Meanwhile 1,8-Dihydroxy-4,5-dinitroanthraquinone inhibits NS2B/3 cleavage in BHK-21 cells at a dose ranging from 4.2 渭M to 432 渭M.
1,8-Dihydroxy-4,5-dinitroanthraquinone (25 渭M; 12-48 h) inhibits extracellular and intracellular dengue virus growth in BHK-21 cells[1].
1,8-Dihydroxy-4,5-dinitroanthraquinone (50 渭M-2 mM; 90 min) inhibits NS2B/3 cleavage, and significantly and dramatically acts function at 100 渭M in BHK-21 cells[1].
[1]. Constant DA, et al. Targeting intramolecular proteinase NS2B/3 cleavages for trans-dominant inhibition of dengue virus. Proc Natl Acad Sci U S A. 2018 Oct 2;115(40):10136-10141.