PE-Cy5.5 is a far-red emitting tandem dye designed based on the principle of fluorescence resonance energy transfer (FRET), and is widely used in multicolor flow cytometry. PE-Cy5.5 consists of phycoerythrin (PE) as the energy donor and the cyanine dye Cy5.5 as the energy acceptor. Upon excitation by blue or green laser light, PE absorbs energy and transfers it to Cy5.5 via FRET, ultimately resulting in Cy5.5 emitting characteristic far-red fluorescence (Ex/Em = 450-500 nm/698 nm) [1].
In Vitro, PE-Cy5.5 conjugates (0.25-5 μg/mL; 30 min at 4 °C) bind strongly and nonspecifically to mouse DEC205-positive CHO/mDEC205, KIT6/mDEC205, and HEK293/mDEC205 cells in proportion to surface mouse DEC205 expression, do not bind to human DEC205-positive cells, and exhibit stronger binding than other Cy5.5-based conjugates[1].
References:
[1]. Park CG, et al. PE-Cy5.5 conjugates bind to the cells expressing mouse DEC205/CD205. J Immunol Methods. 2012;384(1-2):184-190.
In Vitro, PE-Cy5.5 conjugates (0.25-5 μg/mL; 30 min at 4 °C) bind strongly and nonspecifically to mouse DEC205-positive CHO/mDEC205, KIT6/mDEC205, and HEK293/mDEC205 cells in proportion to surface mouse DEC205 expression, do not bind to human DEC205-positive cells, and exhibit stronger binding than other Cy5.5-based conjugates[1].
References:
[1]. Park CG, et al. PE-Cy5.5 conjugates bind to the cells expressing mouse DEC205/CD205. J Immunol Methods. 2012;384(1-2):184-190.