RhoNox-4 is a ferroptosis inhibitor and a fluorescence activation indicator specific to Fe 2+. RhoNox-4 selectively reacts with labile Fe 2+ and oxidizes it to Fe 3+, depleting labile Fe 2+ involved in lipid peroxidation and inhibiting ferroptosis induced by ferroptosis inducers. RhoNox-4 is cell-permeable, accumulates in the endoplasmic reticulum and/or Golgi apparatus, can be used for live-cell imaging and high-throughput screening of subcellular iron status regulators, and is applicable to iron biology research [1] [2] [3] [4].
In Vitro, RhoNox-4 (0.2-12.8 μM; 12 h) acts as a ferroptosis inhibitor in HT1080 cells treated with Erastin, maintaining approximately 80% cell viability at a concentration of 12.8 μM, with an IC50 of approximately 3.5 μM[1][4]. RhoNox-4 (0.2-12.8 μM; 12 h) significantly inhibits RSL-3 -induced ferroptosis in HT1080 cells, and reduces the cell mortality rate by more than 50% at effective concentrations[4]. RhoNox-4 (2 μM; 60 minutes) enables efficient and selective detection of Fe2+[2] in a cell-free HEPES buffer system. RhoNox-4 (1 μM) can effectively penetrate the cell membrane of HepG2 cells and enable live-cell imaging of labile Fe2+[1]. RhoNox-4 (1 μM; 30 minutes) enables efficient live-cell high-throughput screening (HTS) targeting iron homeostasis regulators[2].
References:
[1]. Hirayama T, et al. -oxide-based and trioxolane-based fluorescent probes for monitoring labile iron and labile heme: an update. Journal of clinical biochemistry and nutrition. 2026 Jan;78(1):6-13.
[2]. Hirayama T, et al. High-Throughput Screening for the Discovery of Iron Homeostasis Modulators Using an Extremely Sensitive Fluorescent Probe. ACS sensors. 2020 Sep 25;5(9):2950-2958.
[3]. Tabata R, et al. Systemic Regulation of Iron Acquisition by Arabidopsis in Environments with Heterogeneous Iron Distributions. Plant & cell physiology. 2022 Jun 15;63(6):842-854.
[4]. Kawai K, et al. Inhibition of ferroptosis by -oxide-based fluorescent probes selective oxidation of ferrous ions. Chemical science. 2025 Jun 25;16(25):11240-11245.
In Vitro, RhoNox-4 (0.2-12.8 μM; 12 h) acts as a ferroptosis inhibitor in HT1080 cells treated with Erastin, maintaining approximately 80% cell viability at a concentration of 12.8 μM, with an IC50 of approximately 3.5 μM[1][4]. RhoNox-4 (0.2-12.8 μM; 12 h) significantly inhibits RSL-3 -induced ferroptosis in HT1080 cells, and reduces the cell mortality rate by more than 50% at effective concentrations[4]. RhoNox-4 (2 μM; 60 minutes) enables efficient and selective detection of Fe2+[2] in a cell-free HEPES buffer system. RhoNox-4 (1 μM) can effectively penetrate the cell membrane of HepG2 cells and enable live-cell imaging of labile Fe2+[1]. RhoNox-4 (1 μM; 30 minutes) enables efficient live-cell high-throughput screening (HTS) targeting iron homeostasis regulators[2].
References:
[1]. Hirayama T, et al. -oxide-based and trioxolane-based fluorescent probes for monitoring labile iron and labile heme: an update. Journal of clinical biochemistry and nutrition. 2026 Jan;78(1):6-13.
[2]. Hirayama T, et al. High-Throughput Screening for the Discovery of Iron Homeostasis Modulators Using an Extremely Sensitive Fluorescent Probe. ACS sensors. 2020 Sep 25;5(9):2950-2958.
[3]. Tabata R, et al. Systemic Regulation of Iron Acquisition by Arabidopsis in Environments with Heterogeneous Iron Distributions. Plant & cell physiology. 2022 Jun 15;63(6):842-854.
[4]. Kawai K, et al. Inhibition of ferroptosis by -oxide-based fluorescent probes selective oxidation of ferrous ions. Chemical science. 2025 Jun 25;16(25):11240-11245.