PI-55 |
Catalog No.GC63299
One-Click Copy Product Info
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PI-55 is a selective cytokinin receptor antagonist with an IC₅₀ of approximately 1–5µM for receptor inhibition.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1122579-42-3
Sample solution is provided at 25 µL, 10mM.
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PI-55 is a selective cytokinin receptor antagonist with an IC₅₀ of approximately 1–5µM for receptor inhibition[1]. PI-55 targets Arabidopsis thaliana histidine kinase receptors CRE1/AHK4 and AHK3, and competitively blocks natural cytokinins such as trans-zeatin[2]. Applications of PI-55 are mainly in plant developmental biology and plant stress physiology research, including organogenesis and cadmium stress tolerance[3].
In Arabidopsis thaliana ARR5:GUS reporter assays, PI-55 competitively blocked trans-zeatin with an IC₅₀ of ~1–5µM, reducing cytokinin-induced signaling at 1–50µM for 30min to several hours[4]. In cultures of medicinal plants such as Bulbine natalensis and Rumex crispus, treatment with 0.01–10µM for 7–21 days suppressed shoot induction, promoted root growth, and under cadmium stress improved biomass and antioxidant status[5].
PI-55 at 0.1–10µM for 7–21 days in growth medium decreased ARR5:GUS reporter activity in Arabidopsis thaliana seedlings and modified root development. PI-55 at 0.01–10µM for 14–21 days in MS medium alleviated cadmium-induced growth inhibition in Eucomis autumnalis plantlets, increasing biomass and antioxidant capacity[4]. PI-55 at 0.01–10µM for 14–21 days in MS medium mitigated cadmium stress in Bulbine natalensis and Rumex crispus seedlings, maintaining higher fresh weight and balanced hormone levels[6].
References:
[1] Spíchal, Lukás et al. “The purine derivative PI-55 blocks cytokinin action via receptor inhibition.” The FEBS journal vol. 276,1 (2009): 244-53.
[2] Nisler, Jaroslav et al. “Cytokinin receptor antagonists derived from 6-benzylaminopurine.” Phytochemistry vol. 71,7 (2010): 823-30.
[3] Gemrotová, M., et al. “Seedlings of Medicinal Plants Treated with Cytokinin Antagonist PI‑55 or INCYDE Are Protected against Cadmium Stress.” Plant Growth Regulation vol. 71, 2 (2013): 137–145.
[4] Berková, Veronika et al. “Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome.” Plants (Basel, Switzerland) vol. 9,11 1563. 13 Nov. 2020.
[5] Zahajská, Lenka et al. “Preparation, characterization and biological activity of C8-substituted cytokinins.” Phytochemistry vol. 135 (2017): 115-127.
[6] Werner, Tomás, and Thomas Schmülling. “Cytokinin action in plant development.” Current opinion in plant biology vol. 12,5 (2009): 527-38.
| Kinase experiment [1]: | |
Preparation Method | PI-55 (6-(2-hydroxy-3-methylbenzylamino)purine) was first dissolved in DMSO to obtain a 10mM stock solution and was kept at low temperature in the dark. Immediately before use, the stock was diluted with kinase assay buffer so that the final DMSO content in the reaction did not exceed 1%. Recombinant Arabidopsis thaliana cytokinin receptors (CRE1/AHK4 or AHK3) were incubated in a defined reaction mixture containing ATP and an appropriate peptide or protein substrate. PI-55 was added at the indicated concentrations to evaluate its ability to interfere with cytokinin-dependent receptor activity, typically in the presence of a natural cytokinin such as trans-zeatin. |
Reaction Conditions | 1–50µM, 30min. |
Applications | PI-55 acted as a competitive antagonist of cytokinin receptors. At low micromolar concentrations it suppressed trans-zeatin–induced signaling, with CRE1/AHK4 exhibiting the highest sensitivity. |
| Cultured tissue experiment [2]: | |
Cultured tissue lines | Arabidopsis thaliana tissues such as seedlings, root explants, and ARR5:GUS reporter lines, together with medicinal plant seedlings including Bulbine natalensis and Rumex crispus maintained on MS-based media, are commonly used systems for evaluating the biological activity of PI-55 in plant research. |
Preparation Method | PI-55 was prepared as a 10 mM DMSO stock and diluted into sterile culture medium immediately before use. Explants or seedlings were transferred onto solid or liquid medium supplemented with PI-55 at the required concentrations, while control cultures received the same amount of DMSO without PI-55. The compound was present throughout the cultivation period to continuously modulate cytokinin perception during organogenesis and stress treatments. In experiments combining PI-55 with heavy metal stress, cadmium was added to the medium together with PI-55, and seedlings were grown under controlled light and temperature conditions for several days to weeks. |
Reaction Conditions | 0.1–5µM, 7–21 days. |
Applications | PI-55 suppressed cytokinin signaling, shifting organogenesis toward root formation. At low micromolar concentrations (0.1–10µM, 7–21 days), it reduced cadmium-induced growth inhibition, supported biomass accumulation, maintained balanced hormone activity, and enhanced stress tolerance. |
References: | |
| Cas No. | 1122579-42-3 | SDF | |
| Formula | C13H13N5O | M.Wt | 255.28 |
| Solubility | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9173 mL | 19.5863 mL | 39.1727 mL |
| 5 mM | 783.5 μL | 3.9173 mL | 7.8345 mL |
| 10 mM | 391.7 μL | 1.9586 mL | 3.9173 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 10 reference(s) in Google Scholar.)