Stem Cell Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18309 Dalton. The SCF is purified by proprietary chromatographic techniques.
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Product Documents
Purity:≥ 95% as determined by reducing SDS-PAGE.Appearance:Lyophilized powder
Chemical Properties
Purity
≥ 95% as determined by reducing SDS-PAGE.
Source
Escherichia Coli.
Physical Appearance
Lyophilized powder
Shipping Condition
Shipped at ice.
Synonyms
Kit ligand Precursor; C-kit ligand; SCF; Mast cell growth factor; MGF; SF; KL-1; Kitl; DKFZp686F2250; Hematopoietic growth factor KL; Steel factor.
Amino Acid Sequence
MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.
Apparent Molecular Weight
Approximately 18 kDa, based on SDS-PAGE under reducing conditions Da
Stability
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Biological Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is 0.5915 ng/mL, corresponding to a specific activity is 1.69×106 units/mg.
Formulation
Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
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