GLPBIO AntiFade Mounting Medium is a sealing reagent used to slow the fluorescence quenching. This product can slow the fluorescence quenching of various common fluorescent dyes with simple operation and good anti-fluorescence quenching effect.
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Protocolo (Protocol)
Protocol
For adherent cell samples:
1.1 Discard as much liquid as possible after staining.
1.2 Add one drop of Antifade Mounting Medium to the slide and carefully cover the coverslip containing cells. Be careful to avoid bubbles.
1.3 Detect the fluorescence by fluorescence microscope.
For tissue section samples:
2.1 Discard as much liquid as possible after staining.
2.2 Add one drop of Antifade Mounting Medium to the tissue and carefully cover the coverslip. Be careful to avoid bubbles.
2.3 Detect the fluorescence by fluorescence microscope.
For other samples:
Other samples are processed with reference to adherent cell samples or tissue section samples.
Documentos del producto (Product Documents)
Atributos del producto
Aplicaciones y características
1. This product inhibits the oxidative fading (photobleaching) of fluorescent molecules under light, thereby slowing the decay of fluorescence intensity. However, exposure to light is essential.
2. The product formula avoids interference from common quenchers such as halogen ions and heavy metal ions, ensuring fluorescence stability.
3. This product maintains the activity of the fluorescent dye through an optimized pH and buffer system.
4. This product is compatible with immunofluorescence, in situ hybridization (ISH), super-resolution microscopy, and other assays, making it suitable for a variety of applications, from immediate observation to long-term storage.
Envío
Ship with blue ice.
Condiciones de almacenamiento
-20°C, 1 year
Uso previsto
Solo para uso en investigación. No apto para uso humano.
| Aplicaciones y características | 1. This product inhibits the oxidative fading (photobleaching) of fluorescent molecules under light, thereby slowing the decay of fluorescence intensity. However, exposure to light is essential. 2. The product formula avoids interference from common quenchers such as halogen ions and heavy metal ions, ensuring fluorescence stability. 3. This product maintains the activity of the fluorescent dye through an optimized pH and buffer system. 4. This product is compatible with immunofluorescence, in situ hybridization (ISH), super-resolution microscopy, and other assays, making it suitable for a variety of applications, from immediate observation to long-term storage. |
| Envío | Ship with blue ice. |
| Condiciones de almacenamiento | -20°C, 1 year |
| Uso previsto | Solo para uso en investigación. No apto para uso humano. |
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