(Arg)9,TAMRA-labeled acetate is a TAMRA-labeled, membrane-permeable cationic cell-penetrating polyarginine peptide. (Arg)9,TAMRA-labeled acetate acts as a cell-penetrating peptide for drug delivery [1].
In Vivo, (Arg)9,TAMRA-labeled acetate (20 μM; in solution; overnight; room temperature) shows no membrane adsorption or internal permeation in dechorionated Drosophila melanogaster embryos/larvae, with no viability loss or larval transition observed in treated hosts[1]. (Arg)9,TAMRA-labeled acetate (20 μM; in solution; overnight; room temperature) achieves notable internal permeation in dechorionated-and-dewaxed Drosophila melanogaster embryos, with viability loss unrelated to reagent exposure and all treated hosts remaining in the embryo stage[1].
In Vitro, (Arg)9,TAMRA-labeled acetate (1-5 μM; 3 h, overnight at 37 °C) shows low, dose-dependent permeation into NIH 3T3 mouse embryonic fibroblast cells, with no statistically significant difference in permeation between 37 °C (active endocytosis plus passive diffusion) and 4 °C (passive diffusion only) at 2 μM[1]. (Arg)9,TAMRA-labeled acetate (5 μM; 3 h at 37 °C) shows low permeation into NIH 3T3 mouse embryonic fibroblast cells, as indicated by sparse punctate intracellular fluorescence[1]. (Arg)9,TAMRA-labeled acetate (10 μM; 24 or 48 h at 37 °C) shows no significant cytotoxicity toward NIH 3T3 mouse embryonic fibroblast cells[1]. (Arg)9,TAMRA-labeled acetate (2 μM; 3 h at 37 °C or 4 °C) shows low permeation into H9 human embryonic stem cells, with no statistically significant difference in permeation between 37 °C and 4 °C, and a bimodal fluorescence distribution at 37 °C[1]. (Arg)9,TAMRA-labeled acetate (5 μM; 3 h at 37 °C) shows low permeation into H9 human embryonic stem cells, as indicated by sparse punctate intracellular fluorescence in cell colonies[1]. (Arg)9,TAMRA-labeled acetate (10 μM; 24 or 48 h at 37 °C) shows no significant cytotoxicity toward H9 human embryonic stem cells[1].
References:
[1]. Bowen J, et al. Discovery of Membrane-Permeating Cyclic Peptides via mRNA Display. Bioconjug Chem. 2020;31(10):2325-2338.
In Vivo, (Arg)9,TAMRA-labeled acetate (20 μM; in solution; overnight; room temperature) shows no membrane adsorption or internal permeation in dechorionated Drosophila melanogaster embryos/larvae, with no viability loss or larval transition observed in treated hosts[1]. (Arg)9,TAMRA-labeled acetate (20 μM; in solution; overnight; room temperature) achieves notable internal permeation in dechorionated-and-dewaxed Drosophila melanogaster embryos, with viability loss unrelated to reagent exposure and all treated hosts remaining in the embryo stage[1].
In Vitro, (Arg)9,TAMRA-labeled acetate (1-5 μM; 3 h, overnight at 37 °C) shows low, dose-dependent permeation into NIH 3T3 mouse embryonic fibroblast cells, with no statistically significant difference in permeation between 37 °C (active endocytosis plus passive diffusion) and 4 °C (passive diffusion only) at 2 μM[1]. (Arg)9,TAMRA-labeled acetate (5 μM; 3 h at 37 °C) shows low permeation into NIH 3T3 mouse embryonic fibroblast cells, as indicated by sparse punctate intracellular fluorescence[1]. (Arg)9,TAMRA-labeled acetate (10 μM; 24 or 48 h at 37 °C) shows no significant cytotoxicity toward NIH 3T3 mouse embryonic fibroblast cells[1]. (Arg)9,TAMRA-labeled acetate (2 μM; 3 h at 37 °C or 4 °C) shows low permeation into H9 human embryonic stem cells, with no statistically significant difference in permeation between 37 °C and 4 °C, and a bimodal fluorescence distribution at 37 °C[1]. (Arg)9,TAMRA-labeled acetate (5 μM; 3 h at 37 °C) shows low permeation into H9 human embryonic stem cells, as indicated by sparse punctate intracellular fluorescence in cell colonies[1]. (Arg)9,TAMRA-labeled acetate (10 μM; 24 or 48 h at 37 °C) shows no significant cytotoxicity toward H9 human embryonic stem cells[1].
References:
[1]. Bowen J, et al. Discovery of Membrane-Permeating Cyclic Peptides via mRNA Display. Bioconjug Chem. 2020;31(10):2325-2338.