BG-DBCO (SNAP-DBCO) is a key bifunctional reagent that acts as a "bridge" in the ClickRNA-PROTAC system. BG-DBCO possesses a SNAPTag covalent anchoring handle and a copper-free click chemistry handle: one end covalently locks SNAPTag fusion proteins via BG, while the other end captures azide-modified target protein ligands through copper-free click chemistry (SPAAC), thereby assembling the "exogenous E3 fusion protein" and "POI ligand" into a complete PROTAC ternary complex in living cells [1] [2].
In Vitro, BG-DBCO exhibits biocompatibility when coexisting with SIAH1-SN mRNA and maintains continuous cell survival[1]. BG-DBCO enables tumor-specific assembly of functional PROTAC complexes in trigger mRNA-expressing tumor cells, mediating targeted ubiquitination and degradation of the intended target protein[2].
References:
[1]. Teng X, et al. ClickRNA-PROTAC for Tumor-Selective Protein Degradation and Targeted Cancer Therapy. Journal of the American Chemical Society. 2024 Oct 09;146(40):27382-27391.
[2]. Hou D, et al. Click chemistry-driven tumor theranostics: recent advances, challenges, and future perspectives. Cancer Biol Med. 2026 Mar 12;23(4):480–503.
In Vitro, BG-DBCO exhibits biocompatibility when coexisting with SIAH1-SN mRNA and maintains continuous cell survival[1]. BG-DBCO enables tumor-specific assembly of functional PROTAC complexes in trigger mRNA-expressing tumor cells, mediating targeted ubiquitination and degradation of the intended target protein[2].
References:
[1]. Teng X, et al. ClickRNA-PROTAC for Tumor-Selective Protein Degradation and Targeted Cancer Therapy. Journal of the American Chemical Society. 2024 Oct 09;146(40):27382-27391.
[2]. Hou D, et al. Click chemistry-driven tumor theranostics: recent advances, challenges, and future perspectives. Cancer Biol Med. 2026 Mar 12;23(4):480–503.